Saltar al contenido
Merck
Todas las fotos(1)

Documentos clave

MBD0017

Sigma-Aldrich

Inactivated Escherichia coli

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización

Seleccione un Tamaño

1 ML
249,00 €

249,00 €


Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

Solicitar un pedido a granel

Seleccione un Tamaño

Cambiar Vistas
1 ML
249,00 €

About This Item

Código UNSPSC:
41105500
NACRES:
NA.51

249,00 €


Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

Solicitar un pedido a granel

Nivel de calidad

Formulario

liquid

concentración

>10^8 bacteria/ml

técnicas

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

idoneidad

suitable for restriction endonuclease digests, PCR amplification, Southern blots, and sequencing reactions

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Inactivated Escherichia coli standard can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction. Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E.coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.

Read here how to use our standards to ensure data integrity for your microbiome research.

Aplicación

Inactivated bacteria are provided at >10^8 bacteria/mL concentration in TE buffer pH 8.0. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Características y beneficios

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Forma física

Liquid -The inactivated bacteria is provided at >10^8 bacteria/mL in TE buffer pH 8.0

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

¿No ve la versión correcta?

Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Artículos

DNA standards enhance metagenomics research integrity, offering precise species study and mixed community standards.

Preguntas

1–2 de 2 Preguntas  
  1. Can you provide details on how the bacteria were inactivated? Were they chemically denatured or heat inactivated?

    1 respuesta
    1. The inactivation method is proprietary, and sterility testing is conducted to ensure that complete inactivation has been achieved.

      ¿Le ha resultado útil?

  2. What is the concentration of the bacteria in the suspension?

    1 respuesta
    1. The number of inactivated bacteria per vial is estimated by the CFU count of the untreated control and not by direct count of the final product. The > 10x8 number indicated is an estimation of the number of inactivated bacterial cells per vial.

      ¿Le ha resultado útil?

Revisiones

Sin puntuación

Filtros activos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico