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MAK183

Sigma-Aldrich

PDH Activity Assay Kit

Sufficient for 100 Colorimetric tests

Sinónimos:

Pyruvate Dehydrogenase Activity Assay Kit

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About This Item

Código UNSPSC:
12161503
NACRES:
NA.84

uso

sufficient for 100 colorimetric tests

método de detección

colorimetric

enfermedades relevantes

neurological disorders; cancer

temp. de almacenamiento

−20°C

Información sobre el gen

human ... PDHA1(5160) , PDHA2(5161) , PDHB(5162) , PDHX(8050)
mouse ... PDHA1(18597) , PDHA2(18598) , PDHB(68263) , PDHX(27402)
rat ... PDHA1(29554) , PDHA2(117098) , PDHB(289950) , PDHX(311254)

Descripción general

Pyruvate dehydrogenase (PDH) is a mitochondrial enzyme that catalyzes the conversion of pyruvate to acetyl-CoA and CO2, and also links the tricarboxylic acid (TCA) and glycolysis pathways.[1] The enzyme is inhibited by phosphorylation and activated by dephosphorylation. Mutations in PDH have been linked to pyruvate dehydrogenase deficiency (causing lactic acidosis and neurologic dysfunctions) and Leigh syndrome.[2] PDH has also been implicated in oncogene-induced senescence.[3] PDH measurements can provide insights into metabolic functions and oncogenesis.

Aplicación

PDH Activity Assay Kit has been used to determine the activity of pyruvate dehydrogenase.[4][5][6]

Idoneidad

Suitable for the detection of PDH activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principio

Pyruvate dehydrogenase activity is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product proportional to the enzymatic activity present. One unit of pyruvate dehydrogenase is the amount of enzyme that will generate 1.0 mmole of NADH per minute at 37 °C.

Otras notas

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Solo componentes del kit

Referencia del producto
Descripción
  • PDH Assay Buffer
  • PDH Substrate
  • PDH Developer
  • NADH Standard
  • PDH Positive Control

Pictogramas

CorrosionExclamation mark
GHS05,GHS07

Palabra de señalización

Danger

Frases de peligro

H314,H317

Clasificaciones de peligro

Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

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Certificados de análisis (COA)

Lot/Batch Number
8F02K06790
7F25K06790
3H22K06790
B2I090679V
B2A080679V

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Minfeng Ying et al.
The Journal of biological chemistry, 294(24), 9615-9630 (2019-05-02)
Whether growing cancer cells prefer lactate as a fuel over glucose or vice versa is an important but controversial issue. Labeling of tricarboxylic acid (TCA) cycle intermediates with glucose or lactate isotope tracers is often used to report the relative
The Citric Acid Cycle Oxidizes Two-Carbon Units
Berg J M, et al.
Biochemistry (2002)
Ryan M Bradley et al.
Biochimica et biophysica acta. Molecular and cell biology of lipids, 1863(7), 700-711 (2018-04-09)
Lysophosphatidic acid acyltransferase (LPAAT) δ/acylglycerophosphate acyltransferase 4 is a mitochondrial enzyme and one of five homologues that catalyze the acyl-CoA-dependent synthesis of phosphatidic acid (PA) from lysophosphatidic acid. We studied skeletal muscle LPAATδ and found highest levels in soleus, a
Carine Lindquist et al.
PloS one, 14(9), e0222558-e0222558 (2019-09-25)
A fatty acid analogue, 2-(tridec-12-yn-1-ylthio)acetic acid (1-triple TTA), was previously shown to have hypolipidemic effects in rats by targeting mitochondrial activity predominantly in liver. This study aimed to determine if 1-triple TTA could influence carbohydrate metabolism. Male Wistar rats were
The spectrum of pyruvate dehydrogenase complex deficiency: clinical, biochemical and genetic features in 371 patients.
Patel K P, et al.
Molecular Genetics and Metabolism, 106(3), 385-394 (2012)
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