The kit can detect less than 20 μM of Acetate in various samples. The standard curve ranges from 0 - 10 nmole/well.
MAK086
Acetate Colorimetric Assay Kit
sufficient for 100 colorimetric tests
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Productos recomendados
uso
sufficient for 100 colorimetric tests
método de detección
colorimetric
temp. de almacenamiento
−20°C
Descripción general
Acetate, the ionized form of acetic acid, is a critical metabolite in fatty acid and carbohydrate metabolism. The majority of acetate is utilized as the conjugate to coenzyme A (CoA), acetyl-CoA. Acetyl-CoA is a precursor to fatty acid and cholesterol synthesis and an important starting compound for the citric acid cycle.[1] Acetyl-CoA also serves as a precursor for the synthesis of acetylcholine and as the donor for acetyl groups for post-translational acetylation reactions of histone and non-histone proteins.[2][2] Acetate is generated from the metabolism of carbohydrates, amino acids, fatty acids, and ethanol.
The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.
The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.
Aplicación
Idoneidad
Suitable for the detection of acetate in serum, plasma, animal tissues (liver, kidney, muscle, heart etc.), cell culture (adherent or suspension cells) and food.
Principio
The Acetate Assay kit provides a simple and direct procedure for measuring acetate in a variety of samples. Acetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (450 nm) product proportional to the acetate present.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
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This study investigated the impact of reverse salt flux (RSF) on microbe community and bio-methane production in a simulated fertilizer driven FO-AnMBR system using KCl, KNO
Acetyl Coenzyme A: A Central Metabolite and Second Messenger
Federico P, et al.
Cell Metabolism, 21(6), 805-821 (2015)
A refined genome-scale reconstruction of Chlamydomonas metabolism provides a platform for systems-level analyses.
Imam S, et al.
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A Pichia anomala Strain (P. anomala M1) Isolated from Traditional Greek Sausage is an Effective Producer of Extracellular Lipolytic Enzyme in Submerged Fermentation
Maria P and Emmanuel M P
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Acetyl-CoA the key factor for survival or death of cholinergic neurons in course of neurodegenerative diseases.
Szutowicz A, et al.
Neurochemical Research, 38(8), 1523-1542 (2013)
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What is the minimum detection limit of this assay? What is the quantification range?
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What is the enzymatic reaction and the resulting intermediate substance?
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Initially, acetate is converted to an intermediate in the presence of Acetate Enzyme Mix and Acetate Substrate Mix. This intermediate then reduces a colorless probe to a colored product with strong absorbance at 450 nm. The stated intermediate, considered proprietary, is NADH-based."
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