M9532
Mannitol Dehydrogenase from Leuconostoc mesenteroides
lyophilized powder, ≥60 units/mg protein
Sinónimos:
polyol dehydrogenases, Mannitol:NAD oxidoreductase
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About This Item
Número de CAS:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
Productos recomendados
formulario
lyophilized powder
Nivel de calidad
actividad específica
≥60 units/mg protein
mol peso
136 kDa
temp. de almacenamiento
−20°C
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Descripción general
Mannitol dehydrogenase (MDH) is a NAD-dependent enzyme, obtained from several organisms like, Lactobacillus brevis, Leuconostoc mesenteroides, Absidia glauca and Rhodococcus. MDH obtained from L. mesenteroides exhibits similarity in sequence with the medium-chain dehydrogenase/reductase protein family. It also contains four identical subunits of about 38 kDa in size.
Mannitol dehydrogenase is a cytoplasmic enzyme whose primary role is the regulation of intracellular levels of the sugar alcohol mannitol in plants. It catalyzes the pyridine nucleotide dependent reduction of fructose to mannitol.
The molecular weight of mannitol dehydrogenase is 136 kDa.
Aplicación
Can be used for the production of D-mannitol.
It has been used in a study to assess glucosylglycerol and glucosylglycerate as enzyme stabilizers.
This preparation is useful in the determination of mannitol in urine.
Acciones bioquímicas o fisiológicas
Mannitol dehydrogenase (MDH) helps to catalyze the pyridine nucleotide-dependent oxidation of D-mannitol to D-fructose. Mannitol dehydrogenase (MDH) obtained from L. mesenteroides is highly specific for the conversion fructose and mannitol.
Definición de unidad
One unit will reduce 1.0 μmole of D-fructose per min in the presence of NADH at pH 5.3 at 30°C. One unit of enzyme, as defined above, is equivalent to approximately 1.8 units of enzyme, as described by Lunn et al., one unit will oxidize 1.0 μmole of D-mannitol per min in the presence of NAD at pH 8.6 at 40°C.
Forma física
Lyophilized powder containing buffer salts, potassium phosphate, and dithiothreitol
Nota de preparación
Partially purified
Nota de análisis
Assayed according to the method of Yamanaka, Meth. Enzymol., 41(B), 138 (1975).
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Purification and characterization of a novel mannitol dehydrogenase from Lactobacillus intermedius
Saha, B.
Biotechnology Progress, 20, 537-542 (2012)
Purification and characterization of mannitol dehydrogenase from Aspergillus parasiticus.
Niehaus WG and Dilts RP
Journal of Bacteriology, 151(1), 243-250 (1982)
Thornthan Sawangwan et al.
Biotechnology journal, 5(2), 187-191 (2009-12-01)
Compatible solutes constitute a diverse class of low-molecular-mass organic molecules that are accumulated in high intracellular concentrations in response to the external stress of hyperosmolality or high temperature. Many of these compounds like alpha, alpha-trehalose are well known for their
J Aarnikunnas et al.
Applied microbiology and biotechnology, 59(6), 665-671 (2002-09-13)
The N-terminal amino acid sequences of the intact protein and three tryptic peptides from a 41 kDa protein purified from a commercial mannitol dehydrogenase (MDH) enzyme preparation of Leuconostoc mesenteroides ATCC-9135 were determined. Oligonucleotides deduced from these peptide sequences were
P G Lunn et al.
Clinica chimica acta; international journal of clinical chemistry, 183(2), 163-170 (1989-08-15)
A need for a simple method for the determination of mannitol in urine has arisen because of the use of this monosaccharide in intestinal permeability tests. A rapid spectrophotometric assay for mannitol is presented based on the use of the
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