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M3770

Micrococcus lysodeikticus ATCC No. 4698

suitable for substrate for the assay of lysozyme, lyophilized cells

Sinónimos:

Micrococcus luetus

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NACRES:
NA.54
UNSPSC Code:
12352204
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form

lyophilized cells

Quality Segment

suitability

suitable for substrate for the assay of lysozyme

storage temp.

−20°C

Application

Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles.
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes.

Biochem/physiol Actions

Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.

Analysis Note

Contains polynucleotide phosphorylase.

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

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Este artículo
M050860634LY0100
description

suitable for substrate for the assay of lysozyme, lyophilized cells

description

lyophilized cells

description

solution, dark brown

description

sufficient for 100 tests

suitability

suitable for substrate for the assay of lysozyme

suitability

-

suitability

-

suitability

-

form

lyophilized cells

form

lyophilized cells

form

solution

form

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Clase de almacenamiento

11 - Combustible Solids

¿Qué está pasando?

WGK 3

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

EPI (equipos de protección individual)

Eyeshields, Gloves, type N95 (US)



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Preguntas

  1. From which growth phase are the bacteria harvested?

    1 respuesta
    1. This product is supplied by the ATCC. The growth phase at the time of harvest is not reported by the supplier. In general, in order to maximize production and viability, cells are in log phase or beginning stationary phase at the time of harvest.

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