This product is supplied by the ATCC. The growth phase at the time of harvest is not reported by the supplier. In general, in order to maximize production and viability, cells are in log phase or beginning stationary phase at the time of harvest.
M3770
Micrococcus lysodeikticus ATCC No. 4698
suitable for substrate for the assay of lysozyme, lyophilized cells
Sinónimos:
Micrococcus luetus
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5 G
163,00 €
25 G
611,00 €
100 G
670,60 €
163,00 €
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5 G
163,00 €
25 G
611,00 €
100 G
670,60 €
About This Item
Código UNSPSC:
12352204
NACRES:
NA.54
163,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Formulario
lyophilized cells
Nivel de calidad
idoneidad
suitable for substrate for the assay of lysozyme
temp. de almacenamiento
−20°C
Aplicación
Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. [1] It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles. [2]
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes. [3]
Acciones bioquímicas o fisiológicas
Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.
Calidad
Contains polynucleotide phosphorylase.
Definición de unidad
One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Ghosh et al.
Biochemical engineering journal, 6(1), 19-24 (2000-07-26)
This paper discusses the purification of lysozyme from chicken egg white using hollow-fibre ultrafiltration (30kDa MWCO, polysulphone membrane). Lysozyme is preferentially transmitted through the membrane while the membrane largely retains other egg white proteins. Improvement in system hydrodynamics resulted in
Mathieu Giraudeau et al.
PloS one, 5(10), e13555-e13555 (2010-11-05)
As egg production and offspring care are costly, females should invest resources adaptively into their eggs to optimize current offspring quality and their own lifetime reproductive success. Parasite infections can influence maternal investment decisions due to their multiple negative physiological
Thais Heloisa Vaz Farias et al.
Fish & shellfish immunology, 101, 186-191 (2020-04-05)
Aeromonas hydrophila is responsible for outbreaks of a severe infectious disease in fish farms around the world and is one of the major causes of economic losses to the neotropical fish farmers. This study assessed the induction of immune responses
Ingrid Bourgeois et al.
FEMS microbiology letters, 290(1), 105-113 (2008-11-26)
The nucleotide sequence of atlL, a gene encoding a putative Staphylococcus lugdunensis peptidoglycan hydrolase, was determined using degenerate consensus PCR and genome walking. This 3837-bp gene encodes a protein, AtlL, that appears as a putative bifunctional autolysin with a 29-amino
Jingwei Xie et al.
Journal of colloid and interface science, 317(2), 469-476 (2007-10-20)
A co-axial electrospray process was developed to encapsulate protein-based drugs in biodegradable polymeric microparticles eliminating the key problem faced by other conventional methods of protein encapsulation--the primary emulsion being a major cause for protein denaturation and aggregation. Bovine serum albumin
Protocolos
To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.
This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.
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From which growth phase are the bacteria harvested?
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