L6529
Lipopolysaccharides from Escherichia coli O55:B5
γ-irradiated, BioXtra, suitable for cell culture
Sinónimos:
LPS
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About This Item
Productos recomendados
origen biológico
Escherichia coli (O55:B5)
Nivel de calidad
esterilidad
γ-irradiated
Línea del producto
BioXtra
formulario
lyophilized powder
purificado por
gel-filtration chromatography
técnicas
cell culture | mammalian: suitable
impurezas
<3% Protein (Lowry)
solubilidad
H2O: 5 mg/mL, faintly hazy to hazy
temp. de almacenamiento
2-8°C
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Descripción general
Lipopolysaccharides (LPS) are complex glycolipids that contain several components. Enterobacteriaceae, such as E. coli, have highly conserved lipid A linked to the antigenic O-polysaccharide. Hydrophobic lipid A forms the outer most component that plays a role in the biological properties attributed to endotoxic LPS. A short core oligosaccharide (core OS) is found to be attached to lipid A. Enterobacteria contain an antigenic, repeat-structure O-polysaccharide (O-PS) attached to the distal core OS to form smooth LPS. O-PS in E. coli contains ∼170 distinct antigens that interact with the host immune system. Based on the core OS structures in E. coli, they have been designated K-12 and R1 to R4.
Aplicación
LPS (lipopolysaccharide) from Escherichia coli 055:B5 has been used:
- to stimulate human PBMC to secrete cytokine.
- to stimulate wild type embryonic fibroblasts and mutants for the induction of phosphorylation of p56
- in the measurement of tumor necrosis factor-α levels and nuclear factor-κB p65 activities after stimulation
- in the stimulation of hamster lymphocytes in vitro
- to induce activated dendritic morphology in murine macrophage cell line in a study
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Acciones bioquímicas o fisiológicas
LPS (lipopolysaccharide) is a major constituent of the outer membrane of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. The LPS confers antigenic and endotoxic characters to the bacteria. They are also important in resistance to infection by phages.
Reconstitución
Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.
Otras notas
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
Producto relacionado
Referencia del producto
Descripción
Precios
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 2 Oral
Código de clase de almacenamiento
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.
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