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L2637

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

purified by gel-filtration chromatography

Sinónimos:

LPS

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5 MG
264,00 €
10 MG
604,00 €
25 MG
1270,00 €

264,00 €

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5 MG
264,00 €
10 MG
604,00 €
25 MG
1270,00 €

About This Item

Número de CAS:
93572-42-0
Número CE:
297-473-0
Número MDL:
MFCD00164401
Código UNSPSC:
12352201
NACRES:
NA.25

264,00 €

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origen biológico

Escherichia coli (O55:B5)

Nivel de calidad

200

Formulario

lyophilized powder

purificado por

gel-filtration chromatography

impurezas

<3% Protein

color

white to faint gray

solubilidad

water: soluble

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

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Categorías relacionadas

Descripción general

This product is extracted from E. coli serotype O55:B5 and purified by gel filtration. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/ml and to stimulate equine peritoneal macrophages at 1-100 ng/ml.

Aplicación

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Acciones bioquímicas o fisiológicas

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota de preparación

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Otras notas

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Lipopolysaccharides from Escherichia coli O55:B5, purified by ion-exchange chromatography, TLR ligand tested

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Lipopolysaccharides from Escherichia coli O55:B5, purified by trichloroacetic acid extraction

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Lipopolysaccharides from Escherichia coli O55:B5, γ-irradiated, BioXtra, suitable for cell culture

L2637

Lipopolysaccharides from Escherichia coli O55:B5, purified by gel-filtration chromatography

L2880

Lipopolysaccharides from Escherichia coli O55:B5, purified by phenol extraction

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Lipopolysaccharides from Escherichia coli O55:B5, FITC conjugate

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Lipopolysaccharides from Escherichia coli O55:B5, Ready Made solution, 1 mg/mL

Pictogramas

Skull and crossbones
GHS06

Palabra de señalización

Danger

Frases de peligro

H300

Clasificaciones de peligro

Acute Tox. 2 Oral

Código de clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000431738
0000431738
0000371464
0000371463
0000371465

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John Bienenstock et al.
PloS one, 8(10), e76236-e76236 (2013-10-08)
Human milk oligosaccharides (HMO) are being studied by different groups exploring a broad range of potential beneficial effects to the breastfed infant. Many of these effects have been attributed to a growth promotion effect on certain gut organisms such as
Yunru Ren et al.
Water research, 154, 153-161 (2019-02-21)
Aerosolized reclaimed water can cause inflammatory responses in lung after inhalation, and endotoxin has been identified as the main inducer. Since the effects of disinfection treatments on endotoxins had conflicting results, this study explored the changes of endotoxin activity and
Francisco J Quintana et al.
PloS one, 3(10), e3509-e3509 (2008-10-24)
B-cells integrate antigen-specific signals transduced via the B-cell receptor (BCR) and antigen non-specific co-stimulatory signals provided by cytokines and CD40 ligation in order to produce IgG antibodies. Toll-like receptors (TLRs) also provide co-stimulation, but the requirement for TLRs to generate
Zili Zhai et al.
Inflammation, 36(2), 346-354 (2012-10-12)
The nitrone spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) is commonly used to study free radicals. Due to its free radical trapping properties, DMPO is thought to reduce free radial-mediated oxidative damage and other related cellular responses. The purpose of this study
J Vier et al.
Cell death & disease, 7, e2103-e2103 (2016-02-20)
Neutrophil granulocytes are innate effector cells of the first line of defense against pyogenic bacteria. Neutrophil lifespan is short, is prolonged by pro-inflammatory stimuli, controls functionality of the cells and can determine tissue damage. Experimental analysis of primary neutrophils is
Ver todos los artículos relacionados

Artículos

Lipopolysaccharides for Research – Structure, Function & Applications

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Contenido relacionado

Lipopolysaccharides

Lipopolysaccharide (LPS) is a major component of Gram-negative bacteria, important for microbiological research.

Preguntas

1–4 de 4 Preguntas  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 respuesta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 respuesta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the molecular weight of this product ?

    1 respuesta

    1. Lipopolysaccharides(LPS) are heterogeneous and do not have fully defined molecular weights. In the presence of strong surface active agents, and in the absence of divalent cations, LPS will have molecular weights in the range of 10?20 kDa. Please see the link below to review additional information regarding the nature of LPS, including molecular weight variability:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/research-and-disease-areas/cell-signaling/lipopolysaccharides

      ¿Le ha resultado útil?

  4. Can I use DPBS to dilute? or just use water?

    1 respuesta

    1. This product is tested for solubility at 5 mg/mL in water. Aqueous buffers such as DPBS are expected to be suitable as well. Pease see the link below to review a reference that may be helpful:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5685187/

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