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Key Documents

L2650

Sigma-Aldrich

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

Sinónimos:

SBA, Soybean agglutinin

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.32

origen biológico

Glycine max (soybean)

conjugado

peroxidase conjugate

Análisis

85.00-100.00% (Warburg-Christian)

formulario

lyophilized powder

potencia

<80 μg per mL agglutination activity

peroxidase activity

25-100 units/mg protein

composición

Protein, ~95% modified Warburg-Christian

técnicas

western blot: suitable

Nº de acceso UniProt

P05046

temp. de almacenamiento

−20°C

Información sobre el gen

soybean ... LE1(100818710)

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Aplicación

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Acciones bioquímicas o fisiológicas

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Definición de unidad

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

Forma física

Contains sodium citrate

Nota de preparación

Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Nota de análisis

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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