L0382

Lipase from porcine pancreas

Type VI-S, ≥20,000 units/mg protein, lyophilized powder

• Sinónimos: PPL, Triacylglycerol acylhydrolase, Triacylglycerol lipase
• Número de CAS: 9001-62-1
• Comisión internacional de enzimas: 3.1.1.3 (BRENDA, IUBMB)
• Número CE: 232-619-9
• Número MDL: MFCD00131509
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• tpo: Type VI-S
• Nivel de calidad: 300
• Formulario: lyophilized powder
• actividad específica: ≥20,000 units/mg protein
• composición: Protein, 40-70%
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• Clave InChI: QWZUIMCIEOCSJF-CHHCPSLASA-N

Descripción

Descripción general

Lipase from porcine pancreas (PPL) or porcine pancreatic lipase is a small globular protein with N-terminal α/β type fold. It comprises catalytic triad Ser, Asp, and His residues and a C-terminal β-sandwich domain.[1] PPL belongs to a subclass of the carboxylesterases family.[2]

Aplicación

Lipase from porcine pancreas has been used:

• in lipase inhibition assay with plant extracts[3]
• in in vitro digestion gelatinized starch/fiber mixtures[4]
• with simulated intestinal fluid to mimic the digestive tract condition of by L. fermentum PC1 in fermented oats[5]

Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.

Acciones bioquímicas o fisiológicas

Lipase from porcine pancreas (PPL) catalyzes the hydrolysis of fats (lipids). It plays an essential role in the digestion, transport, and processing of dietary lipids (e.g. triglycerides, fats, oils) in most living organisms.[2] PPL is cost-effective and is useful in biotransformation reactions.[1] It displays high selectivity, and catalytic activity.[1] PPL is also used in the detergent formulation for removing oil stains from clothes.[1]

Lipases catalyze the hydrolysis of esters in aqueous solutions and the synthesis of esters in non-aqueous solutions. They also produce hydroxyl and carboxylic groups through the hydrolysis of ester linkages in poly(ethylene terephthalate).

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Definición de unidad

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.7 at 37 °C using olive oil.

Nota de análisis

Protein determined by biuret.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)