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L0144

Lucifer Yellow CH dipotassium salt

Intracellular staining dye, powder

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A ustedes/SKUDisponibilidadPrecio
25 mg
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207,00 €
100 mg
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331,00 €

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Fórmula empírica (notación de Hill):
C13H9K2N5O9S2
Número CAS:
71206-95-6
Peso molecular:
521.57
NACRES:
NA.77
PubChem Substance ID:
24896249
UNSPSC Code:
12171500
MDL number:
MFCD00013445
Beilstein/REAXYS Number:
5224942
Form:
powder

207,00 €

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Nombre del producto

Lucifer Yellow CH dipotassium salt, powder

form

powder

Quality Segment

100

color

orange

fluorescence

λex 428 nm; λem 540 nm

storage temp.

room temp

SMILES string

[K+].[K+].NNC(=O)NN1C(=O)c2cc(cc3c(N)c(cc(C1=O)c23)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C13H11N5O9S2.2K/c14-10-5-1-4(28(22,23)24)2-6-9(5)7(3-8(10)29(25,26)27)12(20)18(11(6)19)17-13(21)16-15;;/h1-3H,14-15H2,(H2,16,17,21)(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2

InChI key

HCWYSCVNSCIZCN-UHFFFAOYSA-L

General description

Lucifer yellow CH is a fixable dye with an excitation and emission spectrum of 430 nm and 540 nm, respectively.LuciferYellow CH (LYCH) is a small, hydrophilic molecule, and highly fluorescent dye that is used for intracellular staining for the studies conducted on various types of cells.[1] It acts as a marker for the detection of well-established tight junctions in smooth muscles, glia, and interstitial cells of Cajal (ICC).[1]

Application

Lucifer yellow CH dipotassium salt has been used:
  • to visualize cell morphology
  • to assess intercellular communication activity through gap junction by scrape-loading assay
  • as a component of the intracellular solution, which filled the dendrites for the visualization of neuronal morphology
  • in the intracellular solution, to intracellularly label recorded neurons

Features and Benefits

Highly fluorescent dye, useful in marking nerve cells.

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Este artículo
L0259L351050227
Lucifer Yellow CH dipotassium salt powder

Sigma-Aldrich

L0144

Lucifer Yellow CH dipotassium salt

Lucifer Yellow CH dilithium salt fluorescent stain

Sigma-Aldrich

L0259

Lucifer Yellow CH dilithium salt

Lucifer Yellow VS dilithium salt ~85%

Sigma-Aldrich

L3510

Lucifer Yellow VS dilithium salt

D-Luciferin potassium salt ≥98.0% (HPLC)

Sigma-Aldrich

50227

D-Luciferin potassium salt

form

powder

form

powder

form

powder

form

powder or crystals

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

storage temp.

room temp

storage temp.

2-8°C

storage temp.

room temp

storage temp.

−20°C

color

orange

color

faint orange to very dark orange

color

yellow to brown

color

-

fluorescence

λex 428 nm; λem 540 nm

fluorescence

λex 428 nm; λem 540 nm in H2O

fluorescence

λex 428 nm; λem 536 nm in H2O

fluorescence

-

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number

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Preguntas

1–2 de 2 Preguntas  

  1. What is the dye purity for product L0144?

    What is the dye purity for product L0144?

    1 respuesta

    1. At M-Clarity quality level 100, Product L0144 is evaluated based on appearance, infrared spectrum, solubility, and fluorescence specifications. Please refer to the Certificate of Analysis found on the DOCUMENTATION section of the Product Detail Page: https://www.sigmaaldrich.com/product/sigma/l0144#product-documentation

      Using the results on the specification sheet, dye content can be calculated from the testing performed to determine carbon and nitrogen levels.

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  2. What is the stability and shelf life for product L0144, lot SHBN6250 once it has been prepared in a solution?

    1 respuesta

    1. The product is soluble in water at a concentration of 1 mg/mL, producing a clear, yellow-orange colored solution. It is also soluble in water with heat at a concentration of 10 mg/mL, resulting in a slightly hazy orange colored solution. Additionally, a solution stability test revealed that a solution of L0144 in water at a concentration of 1.7 mg/mL, stored in the dark at room temperature for 32 days, showed no detectable change in the UV-VIS spectrum.

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