Saltar al contenido
Merck

Saltar a

I3146

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

Sinónimos:

Human ITS Liquid Media, Insulin-transferrin-sodium selenite

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
Talla/SKUDisponibilidadPrecio
5 mL
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
84,80 €

Acerca de este artículo

NACRES:
NA.75
UNSPSC Code:
12352205

84,80 €


Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad

​
Solicita un pedido personalizado
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle


sterility

sterile-filtered

Quality Segment

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

General description

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells
  • rat fetal testis explants
  • human dental pulp stem cells (DPSC)

Biochem/physiol Actions

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Other Notes

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
I2771I051691077C
description

liquid, sterile-filtered, BioReagent, suitable for cell culture

description

liquid, sterile-filtered, BioReagent, suitable for cell culture

description

10 mg/mL insulin in 25  mM HEPES, pH 8.2, BioReagent, sterile-filtered, suitable for cell culture

description

for research or for further manufacturing use, dry powder

form

liquid

form

liquid

form

solution

form

dry powder

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

500

Quality Level

300

Quality Level

400

Quality Level

300


Clase de almacenamiento

12 - Non Combustible Liquids

¿Qué está pasando?

WGK 1

Punto de inflamación (°F)

Not applicable

Punto de inflamación (°C)

Not applicable

EPI (equipos de protección individual)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos



Preguntas

1–5 de 5 Preguntas  
  1. 培地で希釈した1×Solutionは冷蔵でどのくらい保管できますか?

    1 respuesta
    1. The 100x stock solution of this product has a 2-year shelf life when stored at 2-8°C. However, stability of the solution after dilution or after opening has not been tested.

      ¿Le ha resultado útil?

  2. Can this ITS be diluted in DMEM medium containing phenol red

    1 respuesta
    1. This product is compatible with DMEM containing phenol red.

      ¿Le ha resultado útil?

  3. What should you use to dilute this to a 1X solution?

    1 respuesta
    1. This product can be dissolved in a given target cell culture medium, e.g., non-complex media (MEM, RPMI-1640), to a 1X solution.

      ¿Le ha resultado útil?

  4. What are the product numbers for the stand alone components in I3146?

    1 respuesta
    1. The product numbers for the stand-alone components in I3146 are as follows:
      Insulin – I2643 or I3536 (sterile)
      Apo-Transferrin – T2252
      Sodium Selenite – S5261
      EBBS – E3024

      ¿Le ha resultado útil?

  5. The ITS product #I3146 has been on back order for a while. Is there an alternative with similar components?

    1 respuesta
    1. Based on the Media Supplement Formulation, the closest alternative to product No. I3146 is the I2521 ITS+1 Liquid Media Supplement (100×), which also contains BSA and Linoleic acid.

      ¿Le ha resultado útil?

Revisiones

Sin puntuación

Filtros activos