I2533
Anti-phospho-Insulin Receptor Substrate-1 (pSer312) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
Sinónimos:
Anti-phospho-IRS-1(pSer312)
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About This Item
Productos recomendados
origen biológico
rabbit
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
buffered aqueous glycerol solution
reactividad de especies
human
técnicas
western blot: 1:1000 using stimulated CHO cell line
Nº de acceso UniProt
Condiciones de envío
wet ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
phosphorylation (pSer312)
Información sobre el gen
human ... IRS1(3667)
mouse ... Irs1(16367)
rat ... Irs1(25467)
Descripción general
Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. Phosphorylation of the Tyr1179 residue in the SH-1 negatively regulates insulin. IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. Estrogen receptors (ER) α and β have a strong affinity to IRS-1, an important interaction in breast cancers and medulloblastomas. [1] The expression of IRS-1 in prostate cancer is associated with lower tumor cell invasiveness. Along with ER α, IRS-1 also modulates transcriptional activity involved in the development of breast and prostate cancer. [1] The signalling pathway involving IGF-IR-IRS-1 in cells expressing JCV T-antigen has been reported to affect the proliferation, survival and DNA repair in the cells. Phosphorylation of IRS-1 at Ser312 and Ser616 results in the negative regulation of insulin signalling in humans
Anti-phospho-IRS-1 (pSer312) specifically recognizes human IRS-1 (pSer312). It may react with mouse and rat (100% homology) IRS-1.
Anti-phospho-IRS-1 (pSer312) specifically recognizes human IRS-1 (pSer312). It may react with mouse and rat (100% homology) IRS-1.
Inmunógeno
synthetic phosphopeptide derived from the region of IRS-1 that contains serine 312. This sequence is 100% homologous with mouse and rat IRS-1.
Aplicación
Anti-phospho-IRS-1 (pSer312) antibody may be used for detection by immunoblotting, in stimulated CHO cell line expressing human insulin receptor, at a working dilution of 1:1000.
Forma física
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 containing 50% glycerol, 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Vincent Aguirre et al.
The Journal of biological chemistry, 277(2), 1531-1537 (2001-10-19)
Serine phosphorylation of insulin receptor substrate-1 (IRS-1) inhibits insulin signal transduction in a variety of cell backgrounds, which might contribute to peripheral insulin resistance. However, because of the large number of potential phosphorylation sites, the mechanism of inhibition has been
Joanna Trojanek et al.
Journal of cellular physiology, 206(1), 35-46 (2005-06-21)
JC polyomavirus (JCV), which infects 90% of the human population, is detectable in human tumors. Its early protein, JCV T-antigen, transforms cells in vitro and is tumorigenic in experimental animals. Although T-antigen-mediated transformation involves genetic alterations of the affected cells
Sindhu Saraswathy et al.
Experimental eye research, 196, 108064-108064 (2020-05-23)
This work sought to compare aqueous angiographic segmental patterns with bead-based methods which directly visualize segmental trabecular meshwork (TM) tracer trapping. Additionally, segmental protein expression differences between aqueous angiographic-derived low- and high-outflow human TM regions were evaluated. Post-mortem human eyes
Catia Morelli et al.
Oncogene, 23(45), 7517-7526 (2004-08-20)
Insulin receptor substrate 1 (IRS-1) is a major signaling molecule activated by the insulin and insulin-like growth factor I receptors. Recent data obtained in different cell models suggested that in addition to its conventional role as a cytoplasmic signal transducer
M G Myers et al.
Endocrinology, 132(4), 1421-1430 (1993-04-01)
IRS-1 is a unique cytosolic protein that becomes tyrosine phosphorylated during insulin stimulation of intact cells and immediately associates with the phosphatidylinositol 3'-kinase (PtdIns 3'-kinase). The insulin-like growth factor-I (IGF-I) receptor also mediated the tyrosine phosphorylation of IRS-1 and increased
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