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Merck

I2511

Sigma-Aldrich

IgG from human serum

reagent grade, ≥95% (HPLC), buffered aqueous solution

Sinónimos:

IgG humana

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10 MG
130,00 €

130,00 €


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10 MG
130,00 €

About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

130,00 €


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conjugado

unconjugated

Nivel de calidad

grado

reagent grade

Ensayo

≥95% (HPLC)

Formulario

buffered aqueous solution

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections[1]
Human IgG is purified from normal human serum by precipitation and gel filtration techniques.

Aplicación

Purified human IgG has been used :-
  • as blocking antibody for flow cytometry[2]
  • as negative control in chromatin immunoprecipitation assays[3]
  • to precipitate IgG in human larynx epidermoid carcinoma cells by immunoprecipitation[4]
  • as a standard in enzyme-linked immunosorbent assay[5]

Acciones bioquímicas o fisiológicas

Immunoglobulin G (IgG) regulates the immune responses such as phagocytosis and is also involved in the development of autoimmune diseases.[6] IgG1 regulates complement fixation in mice.[5]

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Kah Teong Soh et al.
Current protocols in cytometry, 75, 7-7 (2016-01-09)
Nucleic acid content can be quantified by flow cytometry through the use of intercalating compounds; however, measuring the presence of specific sequences has hitherto been difficult to achieve by this methodology. The primary obstacle to detecting discrete nucleic acid sequences
In-Kyu Yoon et al.
The American journal of tropical medicine and hygiene, 72(6), 714-718 (2005-06-21)
Volunteers vaccinated with a candidate malaria vaccine containing merozoite surface protein 1(42) (MSP-1(42)) exhibit antibodies to MSP-1(42) that are measured by enzyme-linked immunosorbent assay (ELISA). The purpose of this study was to make a human reference standard for MSP-1(42) antibody
Kentaro Matsuda et al.
The Journal of allergy and clinical immunology, 116(6), 1357-1363 (2005-12-13)
Mouse monoclonal IgE antibodies can promote the survival of mouse bone marrow-derived cultured mast cells and induce the cells to secrete mediators in the absence of known specific antigen. To determine whether human IgE, in the absence of known specific
Sandra S Wright et al.
Journal of pediatric gastroenterology and nutrition, 66(5), 767-772 (2017-12-08)
Elevated granulocyte-macrophage colony-stimulating factor auto-antibodies (GM-CSF Ab) are associated with increased intestinal permeability and stricturing behavior in Crohn disease (CD). We tested for familial association of serum GM-CSF Ab level in CD and ulcerative colitis (UC) families. Serum GM-CSF Ab
Rebeca M Torrente-Rodríguez et al.
PloS one, 12(4), e0175056-e0175056 (2017-04-05)
The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs)

Preguntas

1–2 de 2 Preguntas  
  1. I would like to have the full amino acid sequence of the IgG from product I2511. I cannot find this information in any of the data sheets available online

    1 respuesta
    1. This IgG product exhibits variable domains and has not been sequenced. The material is isolated and purified by precipitation and affinity chromatography. Sequence information on human immunoglobulins is broadly available in published literature. As an example, please see the link below to review the NIH database which includes sequence information:
      https://www.ncbi.nlm.nih.gov/protein/AAA02914.1

      ¿Le ha resultado útil?

  2. What is the shelf life of item A2661 after reconstitution?

    1 respuesta
    1. The stock solution can be stored at -20°C for up to 3 months and can be aliquoted for sampling convenience.

      ¿Le ha resultado útil?

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