The solubility of H9377 has been tested at a concentration of 50 mg/ml in a mixture of 2 parts formic acid to 1 part water, equivalent to a 5% solution. While this may not represent the maximum solubility in the formic acid water mixture, it is the available data. The molecular weight of Hypoxanthine is 136.11 amu, and a 1M solution can be prepared by dissolving 136.11 grams in 1 liter of solvent, provided that there is a suitable solvent for dissolution. This amount can be scaled down to the desired volume. Aliquots should be stored at -20°C for up to 6 months.
H9377
Hypoxanthine
≥99.0%
Sinónimos:
6-Hydroxypurine
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1 G
63,20 €
5 G
129,00 €
25 G
195,00 €
100 G
524,00 €
63,20 €
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1 G
63,20 €
5 G
129,00 €
25 G
195,00 €
100 G
524,00 €
About This Item
Fórmula empírica (notación de Hill):
C5H4N4O
Número de CAS:
Peso molecular:
136.11
Beilstein:
5811
Número CE:
Número MDL:
Código UNSPSC:
41106305
ID de la sustancia en PubChem:
NACRES:
NA.51
63,20 €
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Productos recomendados
origen biológico
synthetic (organic)
Nivel de calidad
Ensayo
≥99.0%
Formulario
powder
mp
>300 °C (lit.)
solubilidad
formic acid: water (2:1): 50 mg/mL, clear to slightly hazy, colorless to yellow
cadena SMILES
O=C1NC=Nc2nc[nH]c12
InChI
1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)
Clave InChI
FDGQSTZJBFJUBT-UHFFFAOYSA-N
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Aplicación
Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.
Hypoxanthine has been used:
- to gavage mice and to evaluate the activity of xanthine oxidase (XO) in pasteurized whole milk in vivo in potassium oxonate (PO) -induced mouse model of hyperuricemia[1]
- as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) medium to cultivate in-vitro cell line (EL-1 cells)[2]
- as a pure standard for the quantification of hypoxanthine in vitreous fluid sample using high-performance liquid chromatography-ultra-violet (HPLC-UV) for the estimation of post-mortem interval[3]
Acciones bioquímicas o fisiológicas
Hypoxanthine acts as an intermediate in purine metabolism. It elicits a vital role as a 5′-base of the anticodon in a few transfer-RNAs. Though similar to guanine (Gua), it is devoid of the exocyclic amino group at C(2) position.[4] It may be used as a substrate to study the specificity and kinetics of hypoxanthine-guanine phosphoribosyl transferases.[5]
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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In our search for novel biocatalysts for the synthesis of nucleic acid derivatives, we found a good candidate in a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK) from Zobellia galactanivorans (ZgHGPRT/AMPK). In this respect, we report for the first time
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Artículos
Uric acid present in animal sera impacts serum-supplemented classical cell culture systems, influencing cell culture performance.
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What are the instructions for preparing a 1M hypoxanthine stock solution?
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