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H1541

Sigma-Aldrich

Anti-HOXC4 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-CP19, Anti-Homeobox 3E, Anti-Homeobox C4

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About This Item

Código UNSPSC:
12352203

origen biológico

rabbit

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

mol peso

antigen ~40 kDa

reactividad de especies

human

concentración

~1.0 mg/mL

técnicas

immunoprecipitation (IP): 5-10 μg/mL using lysates of HEK-293T cells over expressing human HOXC4
indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde fixed HEK-293T cells over expressing human HOXC4
western blot: 5-10 μg/mL using lysates of HEK-293T cells over expressing human HOXC4

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... HOXC4(3221)
mouse ... Hoxc4(15423)
rat ... Hoxc4(24459)

Descripción general

The homeobox C4 (HOXC4) gene is mapped on the human chromosome at 12q13.13.

Especificidad

Anti-HOXC4 (C-terminal) recognizes human HOXC4 (also known as Hox-3E and CP19).

Aplicación

Anti-HOXC4 (C-terminal) antibody produced in rabbit may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Acciones bioquímicas o fisiológicas

Homeobox C4 (HOXC4) transcription factor/protein is involved in inducing the function of activation-induced cytosine deaminase (AID). Upregulation of the HOXC4 gene is due to the estrogen-estrogen receptor complexes. HOXC4 gene is expressed in the nucleus of activated human lymphocytes and the increased expression level is observed with the progression of lymphoid differentiation.

Forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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J van Oostveen et al.
Leukemia, 13(11), 1675-1690 (1999-11-11)
In the last decade it has become clear that homeobox containing genes (HOX genes) not only play a significant role in regulating body formation, but in addition, they are contributing to organization and regulation of hematopoiesis. Modern molecular technologies showed
R Meazza et al.
Blood, 85(8), 2084-2090 (1995-04-15)
We have analyzed the expression of homeoproteins of the HOX family in resting and activated lymphoid cells and in neoplastic lymphoid cell lines by the use of monoclonal antibodies (MoAbs) already shown to react with the homeoproteins HOXA10, HOXC6, and
Seok-Rae Park et al.
Cytokine, 61(2), 608-613 (2012-11-28)
Activation-induced cytidine deaminase (AID) plays a key role in B cell immunoglobulin (Ig) class switch recombination (CSR) and somatic hypermutation (SHM). We have previously reported that the highly conserved homeodomain HoxC4 transcription factor binds to the Aicda (AID gene) promoter
Frank Mendrzyk et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(7 Pt 1), 2070-2079 (2006-04-13)
Pathogenesis of ependymomas is still poorly understood and molecular markers for risk-adapted patient stratification are not available. Our aim was to screen for novel genomic imbalances and prognostic markers in ependymal tumors. We analyzed 68 sporadic tumors by matrix-based comparative
Thach Mai et al.
The Journal of biological chemistry, 285(48), 37797-37810 (2010-09-22)
Estrogen enhances antibody and autoantibody responses through yet to be defined mechanisms. It has been suggested that estrogen up-regulates the expression of activation-induced cytosine deaminase (AID), which is critical for antibody class switch DNA recombination (CSR) and somatic hypermutation (SHM)

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