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GE28-9893-35

Sigma-Aldrich

HiLoad® 16/600 Superdex® 200 pg

Cytiva 28-9893-35, column L × I.D. 60 cm × 16 mm, 34 μm avg. part. size

Sinónimos:

16/600 Superdex 200, HiLoad Superdex 200

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About This Item

Código UNSPSC:
41115700
NACRES:
SB.52

Materiales

bed support (polyamide)
glass column
glass fiber (polypropylene reinforced)

mol peso

10000-600000 Da (globular proteins)

envase

pkg of 1 ea

fabricante / nombre comercial

Cytiva 28-9893-35

Parámetros

<1.0 mL/min flow rate
<5 mL sample volume
0.3 Mpa (42 psi)

lecho volumen

120 mL

columna L × D.I.

60 cm × 16 mm

Matriz

agarose dextran composite
dextran-agarose particle platform
polymeric

grupo activo de la matriz

dextran-agarose phase

tam. prom. pieza

34 μm

cleaning

1-14

Intervalo de trabajo

3-12

técnica de separación

size exclusion (SEC)

Descripción general

HiLoad® 16/600 Superdex® 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Características y beneficios

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Idoneidad

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography, e.g, separate mAbs from dimers and contaminants

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Información legal

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pictogramas

Flame

Palabra de señalización

Warning

Frases de peligro

Código de clase de almacenamiento

3 - Flammable liquids


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Contenido relacionado

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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