GE17-1179-01
Resource™ Q
Cytiva 17-1179-01, pack of 6 mL
Sinónimos:
Resource Q Column
About This Item
Productos recomendados
ligand
quaternary ammonium
descripción
Ion Exchanger Type (value)
envase
pack of 6 mL
fabricante / nombre comercial
Cytiva 17-1179-01
Parámetros
87 psi
lecho tamaño
16 mm × 30 mm
lecho volumen
6 mL
matriz
polystyrene/divinylbenzene
tam. prom. pieza
15 μm
tamaño de partícula
15-15 μm
cleaning
1-14
working range
2-12
idoneidad
suitable for bioprocess medium
Categorías relacionadas
Descripción general
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Aplicación
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
Características y beneficios
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Almacenamiento y estabilidad
Nota de análisis
Información legal
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Código de clase de almacenamiento
3 - Flammable liquids
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
This page shows how to perform a purification of His-tagged membrane proteins.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protocolos
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
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