GE11-0033-99

His GraviTrap^™

Cytiva 11-0033-99

• Sinónimos: His GraviTrap Column
• Código UNSPSC: 12161501
• NACRES: NA.32

Propiedades

• envase: pkg of 10 ea
• fabricante / nombre comercial: Cytiva 11-0033-99
• lecho volumen: 1 mL
• columna D.I.: 15 mm
• matriz: 6% cross-linked agarose
• tamaño de partícula: 45-165 μm
• diámetro promedio: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium)
• working range: 3-12(Ni²⁺-stripped medium.)
• capacidad: ~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.); ~40 mg binding capacity(histidine-tagged protein)
• idoneidad: suitable for bioprocess medium

Descripción

Descripción general

His GraviTrap^™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose^™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap^™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

Características y beneficios

• For fast and simple purification of histidine-tagged* proteins.
• Convenient, prepacked gravity-flow column.
• Allows direct purification of both clarified and unclarified cell lysates.
• High binding capacity; approximately 40 mg of pure protein per column.
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

GraviTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Información de seguridad

• Pictogramas: GHS09
• Palabra de señalización: Warning
• Frases de peligro: H226
• Consejos de prudencia: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Código de clase de almacenamiento: 3 - Flammable liquids