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G4252

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Sinónimos:

S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)

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200 units
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129,00 €
500 units
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254,00 €
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Número CAS:
9033-12-9
UNSPSC Code:
12352204
NACRES:
NA.54
Número CE:
4.4.1.5 (BRENDA, IUBMB)
MDL number:
MFCD00131225
Specific activity:
≥400 units/mg protein

129,00 €

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type

Grade IV

form

buffered aqueous glycerol solution

specific activity

≥400 units/mg protein

foreign activity

glyoxalase II ≤1%

storage temp.

2-8°C

General description

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.

Application

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos.[1] It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.[2]

Biochem/physiol Actions

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.[3]

Physical form

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5

Analysis Note

Protein determined by biuret.

Other Notes

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

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Este artículo
MAK114A7011H6380
Glyoxalase I from Saccharomyces cerevisiae Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Sigma-Aldrich

G4252

Glyoxalase I from Saccharomyces cerevisiae

Glyoxalase I Activity Assay Kit sufficient for 100 tests (UV)

Sigma-Aldrich

MAK114

Glyoxalase I Activity Assay Kit

Alcohol Dehydrogenase from Saccharomyces cerevisiae ≥300 units/mg protein, lyophilized powder (contains buffer salts), Mw 141-151 kDa

Sigma-Aldrich

A7011

Alcohol Dehydrogenase from Saccharomyces cerevisiae

Hexokinase from Saccharomyces cerevisiae lyophilized powder, ≥350 units/mg protein, Protein ≥10 % by biuret

Sigma-Aldrich

H6380

Hexokinase from Saccharomyces cerevisiae

specific activity

≥400 units/mg protein

specific activity

-

specific activity

≥300 units/mg protein

specific activity

≥350 units/mg protein

form

buffered aqueous glycerol solution

form

-

form

lyophilized powder (contains buffer salts)

form

lyophilized powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

foreign activity

glyoxalase II ≤1%

foreign activity

-

foreign activity

-

foreign activity

L-Glutamic dehydrogenase ≤0.05%, Creatine phosphokinase, myokinase, 6-phosphogluconic dehydrogenase and alcohol dehydrogenase ≤0.001%, Glucose-6-phosphate dehydrogenase ≤0.005%, Glutathione reductase ≤0.005%, Phosphoglucose isomerase ≤0.002%

Quality Level

200

Quality Level

-

Quality Level

300

Quality Level

200

type

Grade IV

type

-

type

-

type

-

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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