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Merck

G2625

Sigma-Aldrich

Gelatina from porcine skin

gel strength ~175 g Bloom, Type A

Sinónimos:

Teleostean gelatine

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About This Item

Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.61

origen biológico

Porcine skin

tpo

Type A

formulario

powder

concentración

65-85% (biuret)

técnicas

cell culture | mammalian: suitable

solubilidad

H2O: soluble 50 mg/mL

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Aplicación

This product is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Gelatin has been used in many applications. It has use in coating cell culture to improve attachment of cells, being added to PCR to stabilize Taq DNA, as a blocking reagent in Western blotting, ELISA, and immunochemistry, and as a component of media for species differentiation in bacteriology. As a biocompatible polymer, it has used as a delivery vehicle for release of active biomolecules and in generation of scaffolds for tissue engineering applications. In the pharmaceutical industry, geltan can be used as a suspending and encapsulating agent, among other applications.

Componentes

La gelatina es una mezcla heterogénea de proteínas hidrosolubles de elevados pesos moleculares promedio, presentes en el colágeno. Las proteínas se extraen hirviendo en agua la piel, los tendones, los ligamentos, los huesos, etc.. La gelatina de tipo A procede de tejido curado con ácido. La B procede de tejido curado con cal.

Precaución

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

Nota de preparación

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gel strength of 175.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Discussion of synthetic modifications to gelatin, improving the three-dimensional (3D) print resolution, and resulting material properties.

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