F6008

Ficin from fig tree latex

lyophilized powder

• Sinónimos: Debricin, Ficain, higueroxyl delabarre
• Número de CAS: 9001-33-6
• Comisión internacional de enzimas: 3.4.22.3 (BRENDA, IUBMB)
• Número CE: 232-599-1
• Número MDL: MFCD00081597
• Código UNSPSC: 12352204
• NACRES: NA.54

Propiedades

• origen biológico: fig tree (latex)
• formulario: lyophilized powder
• actividad específica: ≥1.0 units/mg protein
• mol peso: 23.8 kDa
• composición: Protein, 80-100% biuret
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Extinction Coefficient: E^1% = 21.0 (280 nm)
pI: 9.0

Aplicación

Ficin from fig tree latex has been used:

• in the proteolysis of tendon collagen
• in the digestion of Chlorophyta Ulva rigida
• to investigate it effect on protease-activated receptors 2 and 4 activation to induce itch in skin

Acciones bioquímicas o fisiológicas

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

Ficin is effective in disrupting the staphylococcal biofilms making it accessible antibiotics exposure.

Definición de unidad

One unit will produce a ΔA₂₈₀ of 1.0 per min at pH 7.0 at 37°C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).

Forma física

Lyophilized powder containing L-cysteine and trace EDTA

Nota de preparación

This enzyme is soluble in water (10 mg/ml), yielding a clear solution.

Información de seguridad

• Pictogramas: GHS08,GHS07
• Palabra de señalización: Danger
• Frases de peligro: H315,H319,H334,H335
• Consejos de prudencia: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Faceshields, Gloves