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F2555

Sigma-Aldrich

Rabbit Monoclonal ANTI-FLAG® Clone SIGI-25

clone SIG1-25, ascites fluid

Sinónimos:

Anti-ddddk, Anti-dykddddk

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.32

origen biológico

rabbit

Nivel de calidad

200

conjugado

unconjugated

forma del anticuerpo

ascites fluid

tipo de anticuerpo

primary antibodies

clon

SIG1-25, monoclonal

técnicas

immunocytochemistry: 1:125-1:250 using transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein fixed with paraformaldehyde/Triton™ X-100
indirect ELISA: suitable
western blot: 1:250-1:500 using extracts of transiently transfected cells expressing FLAG (sequence at the N-terminus)-tagged protein

isotipo

IgG

secuencia del inmunógeno

DYKDDDDK

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG® peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG® peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG® peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG® peptide is found in both rat and mouse Mg2+ dependent protein b-phosphatase, as well as in the human and bovine enzyme. This rabbit ANTI-FLAG® antibody is produced from the hybridoma SIG1-25. The hybridoma is derived by the fusion of myeloma cells and splenocytes from rabbits immunized with the FLAG® peptide sequence conjugated to KLH.

Especificidad

This monoclonal antibody recognizes the FLAG sequence at the N-terminus

Inmunógeno

FLAG peptide sequence DYKDDDDK

Aplicación

Monoclonal ANTI-FLAG® antibody produced in rabbit has been used in:

  • immunofluorescence
  • western blot analysis
  • immunocytochemistry
  • indirect ELISA

Learn more product details in our FLAG® application portal.

Forma física

Provided as ascites fluid containing 15 mM sodium azide as a preservative.

Información legal

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Qungang Qi et al.
The Journal of biological chemistry, 287(37), 31482-31493 (2012-07-18)
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PLoS ONE, 8(9), e75137-e75137 (2013)
Recombinant lentivirus with enhanced expression of caudal-related homeobox protein 2 inhibits human colorectal cancer cell proliferation in vitro
He S, et al.
Molecular Medicine Reports, 12(2), 1838-1844 (2015)
Reduction of NANOG mediates the inhibitory effect of aspirin on tumor growth and stemness in colorectal cancer
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Cellular Physiology and Biochemistry, 44(3), 1051-1063 (2017)
Jennifer J Banerjee et al.
eLife, 6 (2017-07-01)
Polarity is a shared feature of most cells. In epithelia, apical-basal polarity often coexists, and sometimes intersects with planar cell polarity (PCP), which orients cells in the epithelial plane. From a limited set of core building blocks (e.g. the Par
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