The stability of this Fibronectin solution upon further dilution has not been evaluated. At the prepared 1 mg/mL concentration, the solution is stable for at least 1 year from the date of shipment, when stored at 2-8°C. Please see the following links to review additional information regarding usage and handling of Fibronectin:
Coating protocol
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/fibronectin-product-protocols
General information
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/fibronectin
F1141
Bovine Fibronectin
from bovine plasma, liquid, suitable for cell culture
Sinónimos:
Fibronectina
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| A ustedes/SKU | Disponibilidad | Precio |
|---|---|---|
1 mg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 320,00 € |
2 mg | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 575,00 € |
5 mg | Disponible para enviar HOYdesdeLyon | 1090,00 € |
Acerca de este artículo
Biological source:
bovine plasma
Form:
solution
Technique(s):
cell culture | mammalian: suitable
320,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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Permítanos ayudarleNombre del producto
Fibronectina plasma bovino, solution, sterile-filtered, BioReagent, suitable for cell culture
biological source
bovine plasma
Quality Segment
sterility
sterile-filtered
product line
BioReagent
form
solution
packaging
pkg of 1 mg, pkg of 2 mg, pkg of 5 mg
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
UniProt accession no.
binding specificity
Peptide Source: Collagen
, Peptide Source: Fibrinogen
shipped in
wet ice
storage temp.
2-8°C
Gene Information
bovine ... FN1(280794)
General description
La fibronectina es una proteína ubicua de la matriz extracelular. Es una proteína mosaico compuesta por tres tipos de secuencias de aminoácidos consenso modulares que se repiten. Las fibronectinas pertenecen a una clase de glucoproteínas de elevado peso molecular. La fibronectina celular es expresada por los fibroblastos y la fibronectina plasmática suele ser liberada por los hepatocitos.
Application
La fibronectina (FN) plasmática bovina se ha utilizado en:
- análisis de fibronectina y de adsorción (ELISA (enzimoinmunoanálisis de absorción))
- migración transpocillos
- ensayo de fibronectina
Biochem/physiol Actions
Las fibronectinas participan en la adhesión celular, la organización del citoesqueleto, la transformación oncógena, la migración celular, la fagocitosis, la hemostasia y la diferenciación embrionaria.
Analysis Note
La homogeneidad proteica se evalúa mediante inmunoelectroforesis.
Other Notes
El producto F1141, la fibronectina del plasma bovino es una disolución en TBS 0,05 M a pH 7,5.
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Este artículo | |||
|---|---|---|---|
| description solution, sterile-filtered, BioReagent, suitable for cell culture | description powder, BioReagent, suitable for cell culture | description lyophilized powder, BioReagent, suitable for cell culture | description cell culture derived, ~0.5 mg/mL, sterile-filtered, BioReagent, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile | sterility sterile | sterility sterile-filtered |
| biological source bovine plasma | biological source bovine plasma | biological source human plasma | biological source human fibroblasts |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form powder | form lyophilized powder | form solution |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Clase de almacenamiento
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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How long can I store the diluted solution (20µg/ml) in PBS?
1 respuesta-
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Can I freeze the aliquoted Fibronectin?
1 respuesta-
The recommended storage temperature for this product is 2-8°C. While freezer storage is not required, storing this solution in frozen aliquots at -20°C will not impact the integrity of the protein. Avoid repeated freeze/thaw cycles. The long term stability of this material when stored frozen has not been evaluated and may not result in extended shelf life.
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Hi, I bought a vial with 5mg of Fibronectin (F1141), but so far, I have not been able to find the concentration of this solution. Can you please help me with this info? Thanks a lot in advance!
1 respuesta-
The concentration of this product is 1 mg/mL. This information is reported in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/f1141#product-documentation¿Le ha resultado útil?
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How long can I store fibronectin coated plates, etc?
1 respuesta-
Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
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How do I choose which fibronectin product to use to coat culture surfaces for cell growth?
1 respuesta-
The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
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What is the Department of Transportation shipping information for this product?
1 respuesta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the procedure for coating culture surfaces with fibronectin?
1 respuesta-
To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
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How long can I store the fibronectin solution?
1 respuesta-
The product is good for at least a year at 2-8 °C if kept sterile.
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