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Merck
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E8655

Sigma-Aldrich

Anti-E6AP antibody, Mouse monoclonal

clone E6AP-330, purified from hybridoma cell culture

Sinónimos:

Anti-E2F-6

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About This Item

Número MDL:
MFCD04034520
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

E6AP-330, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~100 kDa

reactividad de especies

human, mouse, rat, monkey

técnicas

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract from 293T cells

isotipo

IgG1

Nº de acceso UniProt

O75461

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... E2F6(1876)
mouse ... E2f6(50496)
rat ... E2f6(313978)

Descripción general

E6AP is an E3 ubiquitin ligase that is expressed by the UB3A gene. Inhibiton or alterations of the UB3A gene may cause a neurological disorder called the Angelman Syndrome. E6AP interacts with E1 and E2 enzymes to mediate ubiquitination of proteins marked for degradation. E6AP also binds with the E6 viral protein present in HPV-infected cells.
Monoclonal Anti-E6AP antibody is a useful tool for the study of E6AP and its function in protein degradation. This antibody is specific for E6AP protein in rat, mouse, human and monkey.

Inmunógeno

human full-length recombinant E6AP.

Aplicación

Monoclonal Anti-E6AP antibody is suitable for use in western blot (1-2 μg/mL using total cell extract from 293T cells), immunocytochemistry, immunoblotting, immunoprecipitation, indirect ELISA and microarray.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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The role of TP53 in Cervical carcinogenesis.
Tommasino, M., et al.
Human Mutation, 21, 307-312 (2003)
Gennaro Altamura et al.
Scientific reports, 8(1), 17529-17529 (2018-12-05)
E6 from high risk human papillomaviruses (HR HPVs) promotes ubiquitination and degradation of p53 tumour suppressor by mediating its binding to ubiquitin ligase E6AP in a ternary complex, contributing to cell transformation in cervical cancer. We have previously shown that
Lilach Simchi et al.
Journal of clinical medicine, 9(5) (2020-05-28)
The UBE3A gene codes for a protein with two known functions, a ubiquitin E3-ligase which catalyzes ubiquitin binding to substrate proteins and a steroid hormone receptor coactivator. UBE3A is most famous for its critical role in neuronal functioning. Lack of
Yi Sun
Cancer biology & therapy, 2(6), 623-629 (2003-12-23)
E3 ubiquitin ligases are a large family of proteins that can be classified into three major structurally distinct types: N-end rule E3s, E3s containing the HECT (Homology to E6AP C-Terminus) domain, and E3s with the RING (Really Interesting New Gene)
Sheng Miao et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(1), 327-333 (2013-01-04)
Pyramidal neurons have a highly polarized dendritic morphology, characterized by one long apical dendrite and multiple short basal dendrites. They function as the primary excitatory cells of the mammalian prefrontal cortex and the corticospinal tract. However, the molecular mechanisms underlying
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