A9917
Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Goat Anti-Mouse IgG (Fab specific)–HRP
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About This Item
Productos recomendados
origen biológico
goat
conjugado
peroxidase conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
formulario
buffered aqueous solution
reactividad de especies
mouse
técnicas
direct ELISA: 1:60,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
western blot: 1:80,000-1:160,000
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Immunoglobulin G (IgG) is a glycoprotein antibody. IgG belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3.
Especificidad
Specificity of the Anti-Mouse IgG (Fab Specific)- Peroxidase is determined by immunoelectrophoresis (IEP), prior to conjugation. By IEP, single precipitation arcs are observed against normal mouse serum, mouse IgG and the Fab fragment of mouse IgG, while no reaction is observed against the Fc fragment of mouse IgG or human IgG. Binds all mouse IgGs.
Inmunógeno
Purified mouse IgG Fab fragment
Aplicación
Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)-Peroxidase antibody has been used in immunofluorescence, ELISA (enzyme-linked immunosorbent assay) and immunoblotting.
Goat Anti-Mouse IgG (Fab specific)-Peroxidase antibody can be used for western blotting assays. The product can also be used for direct ELISA (1:60,000) and IHC (1:150) applications.
Goat Anti-Mouse IgG (Fab specific)-Peroxidase antibody can be used for western blotting assays. The product can also be used for direct ELISA (1:60,000) and IHC (1:150) applications.
Bovine CVEC and human epidermoid carcinoma A431 cell lysates were analyzed by western blot using HRP-conjugated goat anti-mouse IgG Fab specific as the secondary antibody at a 1:3000 dilution.
Acciones bioquímicas o fisiológicas
Immunoglobulin G (IgG) regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. IgG1 regulates complement fixation, opsonization and antibody dependent cell mediated cytotoxicity in mice. IgG participates in hypersensitivity type II and type III.
Otras notas
Antibody adsorbed with human IgG.
Forma física
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.
Nota de preparación
Adsorbed to reduce background staining with human samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Almacenamiento y estabilidad
Store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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