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E7885

Elastase from porcine pancreas

lyophilized powder, suitable for cell culture

Sinónimos:

Elastase from hog pancreas, Pancreatopeptidase E

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Talla/SKUDisponibilidadPrecio
1 mg
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101,00 €
5 mg
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
278,00 €
20 mg
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625,00 €

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Número CAS:
UNSPSC Code:
12352204
NACRES:
NA.75
EC Number:
254-453-6
MDL number:
Número CE:
Specific activity:
≥4 units/mg protein
Biological source:
Porcine

101,00 €


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biological source

Porcine

Quality Segment

form

lyophilized powder

specific activity

≥4 units/mg protein

mol wt

25.9 kDa

packaging

pkg of 1 μmol

technique(s)

cell culture | mammalian: suitable

pH

8

suitability

suitable for

foreign activity

Trypsin ≤50 BAEE units/mg protein

shipped in

dry ice

storage temp.

−20°C

General description

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.

Application

Elastase from porcine pancreas is used for the following applications:
  • Cell culture techniques
  • ELISA techniques
  • Used in assay for elastase inhibitory activity

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.

Packaging

Package size based on protein content

Other Notes

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

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Este artículo
E0127E1250324682
description

lyophilized powder, suitable for cell culture

description

Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein

description

Type I, ≥4.0 units/mg protein

description

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

Porcine

biological source

-

biological source

Porcine pancreas

biological source

-

specific activity

≥4 units/mg protein

specific activity

≥4.0 units/mg protein

specific activity

≥4.0 units/mg protein

specific activity

≥25 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

suspension

form

lyophilized

suitability

suitable for

suitability

-

suitability

-

suitability

-

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

ambient


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pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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