E0760

Endothelial cell growth supplement from bovine pituitary

Name: Endothelial cell growth supplement from bovine pituitary
Brand: SIGMA

Bioreagent, powder, suitable for cell culture

Sinónimos:

ECGS, Endothelial Mitogen

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UNSPSC Code:

12352202

NACRES:

NA.77

Biological source:

bovine pituitary glands

Form:

powder

Impurities:

≤20 EU/vial (LAL test)

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Nombre del producto

Endothelial cell growth supplement from bovine pituitary, ECGS, BioReagent, suitable for cell culture

biological source

bovine pituitary glands

product line

BioReagent

form

powder

potency

2-300 μg/mL ED₅₀/EC₅₀

quality

endotoxin tested

packaging

pkg of 15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

brown to tan

solubility

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

Quality Level

200

Categorías relacionadas

Cell Culture & Analysis

Cell Culture Supplements & Reagents

Growth Factors and Cytokines

Molecular Biology & Functional Genomics

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Este artículo	E2759	E1257	F3133
Sigma-Aldrich E0760 Endothelial cell growth supplement from bovine pituitary	Sigma-Aldrich E2759 Endothelial cell growth supplement from bovine neural tissue	Sigma-Aldrich E1257 Epidermal Growth Factor from murine submaxillary gland	Sigma-Aldrich F3133 Fibroblast Growth Factor from bovine pituitary
biological source bovine pituitary glands	biological source bovine neuroblastoma	biological source mouse submaxillary glands	biological source bovine pituitary glands
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form powder	form lyophilized powder	form lyophilized powder
storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles
packaging pkg of 15 mg	packaging pkg of 15 and 5X15 mg	packaging pkg of 0.1 mg	packaging pkg of 10 and 5X10 μg

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands[1] containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:

as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture[2]
as a supplement in medium 199 for endothelial cell culture[3]
as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12[4]
as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture[5]

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

pictograms

GHS08

signalword

Warning

hcodes

H361fd

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Repr. 2

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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