Unfortunately, the kit components are not sold separately.
DUO94004
Duolink® flowPLA Detection Kit - FarRed
Duolink® PLA kit for Flow Cytometry with FarRed Detection
Sinónimos:
in situ Proximity Ligation Assay, Flowcytometry-PLA, Protein Protein Interaction Kit
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40 TESTS
821,00 €
821,00 €
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40 TESTS
821,00 €
About This Item
Código UNSPSC:
41105331
NACRES:
NA.32
821,00 €
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Línea del producto
Duolink®
técnicas
flow cytometry: suitable
immunofluorescence: suitable
proximity ligation assay: suitable
fluorescencia
λex 644 nm; λem 669 nm
idoneidad
suitable for fluorescence
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
Categorías relacionadas
Descripción general
Duolink® flowPLA Detection Kit contains detection oligonucleotides with a fluorophore (lex = 644 nm/lem = 669 nm).
Especificidad
Far Red Fluorescence Detection Reagents
Use appropriate laser for λex 644 nm excitation
Use appropriate filter for λem 669 nm emission
Use appropriate laser for λex 644 nm excitation
Use appropriate filter for λem 669 nm emission
Aplicación
Based on proximity ligation assay (PLA), the Duolink® PLA Technology allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells.
Duolink® flowPLA Detection Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest, a pair of PLA probes (one 100RXN PLUS and one 100RXN MINUS), wash buffer, and a Duolink® flowPLA Detection Kit. The flowPLA Kits are available with 5 different fluorophores: Violet, Red, Green, Orange, or FarRed. The flowPLA Kits contain all the necessary reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, primary antibodies, and corresponding PLA Probes.
Follow the Duolink® PLA Flow Cytometry Protocol to use this product.
Visit our Duolink® PLA Flow Cytometry page on how to run a Duolink® flow experiment, applications, troubleshooting, and more.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Duolink® flowPLA Detection Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest, a pair of PLA probes (one 100RXN PLUS and one 100RXN MINUS), wash buffer, and a Duolink® flowPLA Detection Kit. The flowPLA Kits are available with 5 different fluorophores: Violet, Red, Green, Orange, or FarRed. The flowPLA Kits contain all the necessary reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, primary antibodies, and corresponding PLA Probes.
Follow the Duolink® PLA Flow Cytometry Protocol to use this product.
Visit our Duolink® PLA Flow Cytometry page on how to run a Duolink® flow experiment, applications, troubleshooting, and more.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects
View full Duolink® product list
Duolink® flowPLA Detection Kit–FarRed has been used in in situ proximity ligation assay:
- to detect interaction and complex formation between cellular retinoic acid binding protein 1 (Crabp1) and the components of rapidly accelerated fibrosarcoma (Raf) kinase- MAPK-Erk kinase (Mek) signaling pathway[1]
- to study protein interaction in human cultured MOLT-4 cells and HeLa cells[2]
- to visualize Beclin-1 protein interaction with 14-3-3t in neurons[3]
- to study protein interactions in graft endothelial cells[4]
Características y beneficios
- Analyze protein protein interactions with flow cytometry readout
- Analyze cell populations with Proximity Ligation Assay
- Increased sensitivity due to rolling circle amplification for low abundant targets
- No overexpression or genetic manipulation required
- Relative quantification possible
- Works with any flow cytometer instrumentation
- Easy to follow flexible protocol
- Publication-ready results
Componentes
This product is comprised of the following:
See datasheet for more information.
- 5x Detection Solution - FarRed (DUO84004)
- 5x Ligation Buffer (DUO82009)
- 5x Amplification Buffer (DUO82050)
- Ligase (1U/μL)
- Polymerase (10U/μL)
See datasheet for more information.
Información legal
Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
10 - Combustible liquids
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Artículos
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
General tips and tricks for proper experiment execution, aid in identifying potential problems, and provide solutions to ensure a successful Duolink® PLA experiment for flow cytometry.
Duolink® PLA kit enhances flow cytometry for detecting protein interactions accurately.
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Is it possible to just buy the 5x Detection Solution separately?
1 respuesta-
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Can I use this kit in conjunction with staining for other markers? If so, would you recommend staining with the PLA kit first, followed by staining with other primary-conjugated antibodies?
1 respuesta-
It is possible to counterstain after the Duolink in situ amplification. It is recommended to apply the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Please see the protocol below:
https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/protein-and-nucleic-acid-interactions/duolink-counterstain¿Le ha resultado útil?
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Hello, can I also dilute the 5 x detection stock in something else than high purity water? Maybe PBS?
1 respuesta-
It is not recommended to use any other solvent to dilute the 5x detection stock due to the sensitivity of this reaction. The Duolink kits have been optimized for use under the conditions described. Deviation from the protocol may not offer the best results. Please see the link below to view the full product information for the Duolink PLAFlow kits:
https://www.sigmaaldrich.com/technical-documents/technical-article/protein-biology/protein-and-nucleic-acid-interactions/duolink-pla-flow-cytometry-kits¿Le ha resultado útil?
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