DUO92001
Duolink® In Situ PLA® Probe Anti-Mouse PLUS
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
30 reactions | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 360,00 € |
100 reactions | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 467,00 € |
Acerca de este artículo
360,00 €
biological source
donkey (polyclonal)
Quality Segment
antibody form
affinity purified immunoglobulin (secondary antibody)
antibody product type
primary antibodies
product line
Duolink®
species reactivity
mouse
technique(s)
immunofluorescence: suitable, proximity ligation assay: suitable
suitability
suitable for brightfield, suitable for fluorescence
shipped in
wet ice
storage temp.
2-8°C
Application
This product can be applied to both the Duolink® In Situ Fluorescence Protocol and the Duolink® In Situ Brightfield Protocol depending on the detection reagents used.
Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.
To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection.
PLA probe anti-Mouse reacts with whole molecule Mouse IgG and the light chains of other Mouse immunoglobulin?s. The PLA probe anti-Mouse may cross-react with Rat antibodies, but has minimal cross reactivity with bovine, chicken, goat, guinea pig, Syrian hamster, horse, human, rabbit, and sheep serum proteins. A MINUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
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Features and Benefits
- No overexpression or genetic manipulation required
- High specificity (fewer false positives)
- Single molecule sensitivity due to rolling circle amplification
- Relative quantification possible
- No special equipment needed
- Quicker and simpler than FRET
- Increased accuracy compared to co-IP
- Publication-ready results
Other Notes
- 5x PLA Probe Anti-Mouse PLUS - Donkey anti-mouse secondary antibody conjugated to oligonucleotide PLUS
- 1x Blocking Solution - Reagent for blocking of the sample
- 1x Antibody Diluent - For dilution of PLA probes and primary antibodies
Legal Information
1 of 1
Este artículo | |||
|---|---|---|---|
| description - | description - | description Affinity purified Donkey anti-Rabbit IgG (H+L) | description Affinity purified Donkey anti-Goat IgG (H+L) |
| biological source donkey (polyclonal) | biological source donkey (polyclonal) | biological source donkey (polyclonal) | biological source donkey (polyclonal) |
| species reactivity mouse | species reactivity rabbit | species reactivity rabbit | species reactivity goat |
| antibody form affinity purified immunoglobulin (secondary antibody) | antibody form affinity purified immunoglobulin (secondary antibody) | antibody form affinity purified immunoglobulin (secondary antibody) | antibody form affinity purified immunoglobulin (secondary antibody) |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| technique(s) proximity ligation assay: suitable, immunofluorescence: suitable | technique(s) proximity ligation assay: suitable, immunofluorescence: suitable | technique(s) proximity ligation assay: suitable, immunofluorescence: suitable | technique(s) proximity ligation assay: suitable, immunofluorescence: suitable |
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Palabra de advertencia
Warning
Códigos de peligro
Indicaciones de precaución
Hazard Classifications
Aquatic Chronic 2 - Skin Sens. 1
Clase de almacenamiento
12 - Non Combustible Liquids
WGK
WGK 3
Punto de inflamación (°F)
Not applicable
Punto de inflamación (°C)
Not applicable
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