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D4418

Sigma-Aldrich

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

Sinónimos:

DAB, DAB tablets, Immunohistology substrate

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About This Item

Número de CAS:
91-95-2
Número MDL:
MFCD00007725
Código UNSPSC:
12352204
ID de la sustancia en PubChem:
24893872
NACRES:
NA.83

product name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 15 mL

formulario

tablet

solubilidad

water: 1 tablet/5 mL

temp. de almacenamiento

−20°C

cadena SMILES

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

Clave InChI

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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Categorías relacionadas

Aplicación

Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitución

Each tablet set dissolved in 15 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Información legal

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Palabra de señalización

Danger

Clasificaciones de peligro

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Órganos de actuación

Respiratory system

Código de clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Listados normativos

Los listados normativos se proporcionan para los productos químicos principalmente. Para los productos no químicos sólo se puede proporcionar información limitada. Si no hay ninguna entrada, significa que ninguno de los componentes está en la lista. Es obligación del usuario garantizar el uso seguro y legal del producto.

EU REACH Annex XVII (Restriction List)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Erin N Scully et al.
Behavioural brain research, 331, 151-158 (2017-05-17)
Zebra finches (Taeniopygia guttata) are sexually dimorphic songbirds, not only in appearance but also in vocal production: while males produce both calls and songs, females only produce calls. This dimorphism provides a means to contrast the auditory perception of vocalizations
F Gualtieri et al.
Scientific reports, 9(1), 7129-7129 (2019-05-11)
In the mammalian brain, adult hippocampal neurogenesis (AHN) is suppressed by chronic stress, primarily at the ventral pole of the hippocampus. Based upon anatomy, we hypothesise that the caudal pole of the avian Hippocampal Formation (HF) presents a homologous subregion.
Huiming Yang et al.
Nature communications, 11(1), 2582-2582 (2020-05-24)
Polyglutamine expansion in proteins can cause selective neurodegeneration, although the mechanisms are not fully understood. In Huntington's disease (HD), proteolytic processing generates toxic N-terminal huntingtin (HTT) fragments that preferentially kill striatal neurons. Here, using CRISPR/Cas9 to truncate full-length mutant HTT
Monika Verma et al.
Toxicon : official journal of the International Society on Toxinology, 139, 58-65 (2017-09-19)
Anthrax associated causalities in humans and animals are implicated mainly due to the action of two exotoxins that are secreted by the bacterium Bacillus antharcis during the infection. These exotoxins comprise of three protein components namely protective antigen (PA), lethal
Ya-Chao Wang et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, 37(3), 980-993 (2016-05-14)
The NMDA antagonist memantine preferentially inhibits extrasynaptic NMDA receptors, which are overactivated upon stroke and thought to disturb neuroplasticity. We hypothesized that memantine enhances post-ischemic neurological recovery, brain remodeling, and plasticity. C57BL6/j mice were exposed to intraluminal middle cerebral artery
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Protocolos

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Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Cell Lysis and Protein Extraction for Western Blotting

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

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