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Merck

CWA-1045

Sigma-Aldrich

Anti-Human CD10 (MEM-78) ColorWheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, sold separately)

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

forma del anticuerpo

purified antibody

tipo de anticuerpo

primary antibodies

clon

MEM-78, monoclonal

Línea del producto

ColorWheel®

Formulario

lyophilized

mol peso

calculated mol wt 88.51 kDa

reactividad de especies

human

envase

antibody small pack of 25 μL

características de los productos alternativos más sostenibles

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

técnicas

flow cytometry: suitable

isotipo

IgG1

secuencia del epítopo

Extracellular domain

Nº de acceso Protein ID

Nº de acceso UniProt

compatibilidad

for use with ColorWheel® Dyes (Required, sold separately)

categoría alternativa más sostenible

Condiciones de envío

ambient

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... MME(4311)

Descripción general

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Especificidad

Clone MEM-78 is a mouse monoclonal antibody that detects human Naprilysin (CD10). It targets an epitope within the extracellular domain.

Inmunógeno

NALM-6 human pre-B cells.

Aplicación

Quality Control Testing:

Evaluated by Flow Cytometry in NALM-6 cell line.

Flow Cytometry Analysis: Staining of one million human NALM-6 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1045, Anti-Human CD10 (MEM-78) ColorWheel® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel® Antibody-Ready Phycoerythrin (PE) Dye an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibilidad

Requires ColorWheel® Dye (Sold Separately)

Descripción de destino

Neprilysin (UniProt: P08473; also known as EC:3.4.24.11, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 24.11, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10) is encoded by the MME (also known as EPN) gene (Gene ID: 4311) in human. CD10 is a single-pass type II membrane glycoprotein of the peptidase M13 family that has a cytoplasmic domain (aa 2-28), a transmembrane domain (aa 29-51), and a long extracellular domain (aa 52-750). It is highly expressed on epithelial cells of lung, intestine, kidney, breast, placenta. CD10 displays thermolysin-like specificity, but is almost confined to acting on polypeptides of up to 30 amino acids. It plays an important role in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. It is also reported to degrade atrial natriuretic factor as well as brain natriuretic factor. Neprilysin is also reported to protect neurons against amyloid peptide toxicity. It is considered as a cell surface marker in the diagnosis of acute lymphocytic leukemia. Glycosylation of CD10 at Asn-628 is shown to be essential for its surface expression and neutral endopeptidase activity. Mutations in MME gene have been linked to Charcot-Marie-Tooth disease that is characterized by progressive weakness and atrophy of peroneal muscles and distal muscles of the arms. (Ref.: Higuchi, Y., et al. (2016). Ann. Neurol. 79(4); 659-672; Wisner, A., et al. (2006). Proc. Natl. Acad. Sci. USA. 103(47); 17979-17984).

Forma física

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitución

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Almacenamiento y estabilidad

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Información legal

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

13 - Non Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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