CS1010 kit measures total HDAC activity in cellular, nuclear, or purified enzyme extracts.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/401/235/cs1010bul.pdf
CS1010
Histone Deacetylase Assay Kit, Fluorometric
sufficient for 100 assays (96 well plates)
Sinónimos:
HDAC Assay Kit
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| Talla/SKU | Disponibilidad | Precio |
|---|---|---|
1 kit | Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad | 1020,00 € |
Acerca de este artículo
UNSPSC Code:
12161503
EC Number:
200-664-3
NACRES:
NA.84
1020,00 €
Póngase en contacto con nuestro Servicio de Atención al Cliente para disponibilidad
Servicio técnico
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sufficient for 100 assays (96 well plates)
Quality Segment
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... HDAC1(3065)
Application
The Histone Deacetylase Assay Kit provides a simple method for the detection of HDAC activity based on a two-step enzymatic reaction. The substrate for this enzymatic assay is a substituted peptide with an acetylated lysine residue and a bound fluorescent group. The first step of the reaction is deacetylation of the acetylated lysine side chain by the HDAC containing sample (HeLa cell extract, purified enzyme, etc.). The second step is the cleavage of the deacetylated substrate by the developer solution and the release of the free highly fluorescent group. The measured fluorescence is directly proportional to the deacetylation activity of the sample.
Biochem/physiol Actions
Histone deacetylases (HDACs) are enzymes that remove acetyl groups from histone proteins. HDACs are important regulators of gene expression and genome function. They are implicated in a number of human disease states, namely several cancers, neurological disorders, and aging. Therefore, HDAC are relevant key targets for therapeutic intervention. Moreover, histone deacetylase inhibitors have been shown to serve as anti-tumor agents.
Features and Benefits
The kit includes all the reagents required for the fast and easy measurement of HDAC activity in cell or nuclear extracts, or with purified enzyme preparations. In addition, the kit provides HeLa cell lysate as a source of HDAC activity for inhibitor screening or as a positive control, a HDAC inhibitor (Trichostatin A), and a standard to enable activity quantitation.
Analysis Note
The Histone Deacetylase Assay Kit has been tested on HeLa, HEK 293T, NIH 3T3, and U 973 cell extracts.
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Este artículo | |||
|---|---|---|---|
| description sufficient for 100 assays (96 well plates) | description recombinant, expressed in baculovirus infected insect cells, ≥50% (SDS-PAGE) | description - | description ≥95% (HPLC) |
| Gene Information human ... HDAC1(3065) | Gene Information human ... HDAC1(3065) | Gene Information human ... HDAC1(3065) | Gene Information - |
| Quality Level 200 | Quality Level - | Quality Level 100 | Quality Level - |
| usage sufficient for 100 assays (96 well plates) | usage - | usage - | usage - |
| storage temp. −20°C | storage temp. −70°C | storage temp. −20°C | storage temp. −70°C |
| shipped in dry ice | shipped in dry ice | shipped in wet ice | shipped in dry ice |
Solo componentes del kit
Referencia del producto
Descripción
- Assay Buffer 20 mL
- Developer Solution 1.5 mL
- HDAC Inhibitor (Trichostatin A), 1 mM 50 μL
- HDAC Substrate, 20 mM 50 μL
- HeLa Cell Lysate 500 μL
- Standard (non-acetylated substrate), 4 mM 50 μL
Clase de almacenamiento
10 - Combustible liquids
Punto de inflamación (°F)
188.6 °F - closed cup
Punto de inflamación (°C)
87 °C - closed cup
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which enzyme mainly present in this assay kit. is it only HDAC 1?
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