The B9059 BACE-1 product included in the kit, serves as a positive control. The Technical Bulletin notes that the concentration of this item is approximate 3 units/microliter. This material has not been tested for purity. Therefore, a molar concentration cannot be provided.
CS0010
β-Secretase (BACE1) Activity Detection Kit (Fluorescent)
1 kit sufficient for 250 reactions
Sinónimos:
BACE1 Activity Detection Kit (Fluorescent)
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Acerca de este artículo
NACRES:
NA.84
UNSPSC Code:
12161503
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Servicio técnico
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Permítanos ayudarleQuality Level
usage
kit sufficient for 250 reactions
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... BACE1(23621)
Categorías relacionadas
1 of 4
Este artículo | |||
|---|---|---|---|
| Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) | Gene Information human ... BACE1(23621) |
| usage kit sufficient for 250 reactions | usage - | usage - | usage - |
| Quality Level 300 | Quality Level - | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. - | storage temp. - |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Application
The kit provides all the reagents required for an efficient detection of BACE1 activity. It contains an enzyme to be used for screening for potential BACE1 inhibitors. The assay is based on the fluorescence resonance energy transfer (FRET) method in which the fluorescence signal enhancement is observed after substrate cleavage by BACE1.
Biochem/physiol Actions
BACE1 is a transmembrane protease responsible for the β site cleavage of the amyloid precursor protein (APP) to produce amyloid β peptide (Aβ). The accumulation of Aβ in the brain is a primary cause for the progression of Alzheimer′s. BACE1 is a target for inhibitor drug discovery.
Solo componentes del kit
Referencia del producto
Descripción
- Fluorescent Assay Buffer 50 mL
- Stop Solution 15 mL
- Substrate (MOCA-SEV-NL-DAEFR-DNP-RR) 500 μL
- Assay Standard 140 μL
- BACE1 (β−Secretase) 300 units 100 μL
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 3
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Lucas J Gutiérrez et al.
Journal of biomolecular structure & dynamics, 37(1), 229-246 (2018-01-06)
We report in this work new substituted aminopyrimidine derivatives acting as inhibitors of the catalytic site of BACE1. These compounds were obtained from a molecular modeling study. The theoretical and experimental study reported here was carried out in several steps:
Piyoosh Sharma et al.
European journal of medicinal chemistry, 167, 510-524 (2019-02-21)
The multitarget-directed strategy offers an effective and promising paradigm to treat the complex neurodegenerative disorder, such as Alzheimer's disease (AD). Herein, a series of N-benzylpiperidine analogs (17-31 and 32-46) were designed and synthesized as multi-functional inhibitors of acetylcholinesterase (AChE) and
Fluoro-benzimidazole derivatives to cure Alzheimer's disease: In-silico studies, synthesis, structure-activity relationship and in vivo evaluation for β secretase enzyme inhibition.
Sayyad Ali et al.
Bioorganic chemistry, 88, 102936-102936 (2019-05-06)
Maricarmen Hernández-Rodríguez et al.
Molecular neurobiology, 57(9), 3979-3988 (2020-07-09)
The increase of amyloid beta (Aβ) release and hyperphosphorylation of Tau protein represents the main events related to Alzheimer's disease (AD). Furthermore, the sporadic type represents the most common form of AD. Therefore, the establishment of a non-transgenic animal model
Vijay K Nuthakki et al.
Bioorganic chemistry, 90, 103062-103062 (2019-06-21)
Alkaloids have always been a great source of cholinesterase inhibitors. Numerous studies have shown that inhibiting acetylcholinesterase as well as butyrylcholinetserase is advantageous, and have better chances of success in preclinical/ clinical settings. With the objective to discover dual cholinesterase
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what is the concentration of BACE1 enzyme in micromolar?
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