CMC0008

STEADY Chemically Competent Cells

Escherichia coli, rod shaped

• Código UNSPSC: 12352200

Propiedades

• Nombre del producto: STEADY Chemically Competent Cells, for plasmid production using unstable DNA
• origen biológico: Escherichia coli
• grado: Molecular Biology; for molecular biology
• Formulario: buffered aqueous solution
• modo de crecimiento: adherent or suspension
• morfología: rod shaped
• técnicas: microbiological culture: suitable
• transformación celular: competent cell type: chemically competent; transformation efficiency: >1 x 10⁸ cfu/μg
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C

Descripción

Descripción general

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Características y beneficios

• Clone unstable sequences with reliable results
• Available as chemically competent or electrocompetent cells
• Your choice of two packaging formats
• High efficiency: over 1 × 10⁸ cfu/μg (chemi) or 1 × 10¹⁰ cfu/μg (electro)
• Excellent value

Componentes

• STEADY chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids