CLLS1127
DLD1 Cells TP53 -/-
human male colon (Source Disease: Colorectal adenocarcinoma)
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About This Item
Código UNSPSC:
41106514
NACRES:
NA.81
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Nombre del producto
DLD1 Cells TP53 -/-,
origen biológico
human male colon (Source Disease: Colorectal adenocarcinoma)
Nivel de calidad
Nº de acceso OMIM
temp. de almacenamiento
−196°C
Información sobre el gen
human ... TP53(7157)
Descripción general
DLD1 Cells TP53-/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN double knock out modification.
This product corresponds to ATCC Cat. No. CCL-221.
This product corresponds to ATCC Cat. No. CCL-221.
Aplicación
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1.3-1.10
The base medium for this cell line is RPMI, Catalog No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Catalog No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Catalog No. S8636, to a final concentration of 1 mM.
The cell freezing medium is DMSO 1X (Catalog Number C6164).
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1.3-1.10
The base medium for this cell line is RPMI, Catalog No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Catalog No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Catalog No. S8636, to a final concentration of 1 mM.
The cell freezing medium is DMSO 1X (Catalog Number C6164).
Características y beneficios
Zinc finger nuclease (ZFN) double knock out on chromosome 17p13.1
The DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
The DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Calidad
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, and PCR assay for cell line species confirmation.
Cláusula de descargo de responsabilidad
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
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