CHROP

Imprint^® Ultra Chromatin Optimization Kit

Kit designed to optimize sonication parameters for ChIP experiments

• NACRES: NA.54
• UNSPSC Code: 41116158

Propiedades

• Quality Segment: 200

Descripción

General description

The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.

Other Notes

Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 10⁷ cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.

Legal Information

Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

Los componentes del kit también están disponibles por separado

• I8896

  IGEPAL^® CA-630, Molecular Biology

  SDS
• S5150

  Sodium chloride solution, 5 M in H₂O, BioReagent, Molecular Biology

  SDS
• W4502

  Water, Nuclease-Free Water, for Molecular Biology

  SDS
• A8456

  4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)

  SDS
• P8340

  Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

  SDS
• R4642

  Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

  SDS

Información de seguridad

• pictograms: GHS02,GHS03,GHS08,GHS05,GHS07,GHS09
• signalword: Danger
• hcodes: H226,H271,H290,H302,H314,H334,H336,H410
• pcodes: P210 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
• target_organs: Central nervous system
• Clase de almacenamiento: 5.1A - Strongly oxidizing hazardous materials
• flash_point_f: 100.4 °F
• flash_point_c: 38 °C