CHROP
Imprint® Ultra Chromatin Optimization Kit
Kit designed to optimize sonication parameters for ChIP experiments
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15 REACTIONS
332,00 €
332,00 €
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15 REACTIONS
332,00 €
About This Item
Código UNSPSC:
41116158
NACRES:
NA.54
332,00 €
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Descripción general
The Imprint Ultra Chromatin Optimization Kit provides reagents needed to optimize chromatin sonication/shearing conditions before performing ChIP. It is imperative that chromatin is completely sheared into fragments with a peak around 500 base pairs (bp) for ChIP-qPCR assays, and < 500 bp for ChIP-Seq and ChIP-chip, for maximum specificity and resolution, and to ensure low background. For higher resolution applications like genome-wide location analysis by ChIP-chip or ChIP-Seq, sonication of input chromatin to below 500 bp, with a peak around 250 bp, is critical.
Componentes
Sufficient reagents are provided to perform three sets of chromatin sonication optimization experiments. Each set would include five different conditions for sonication to optimize the parameters. Reagents are provided for a total of 15 reactions, including preparation of cell lysates from 107 cells/reaction, nuclei lysis, sonication, cross-link reversal and DNA purification.
Información legal
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- I8896IGEPAL® CA-630, for molecular biologySDS
- S5150Sodium chloride solution, 5 M in H2O, BioReagent, for molecular biology, suitable for cell cultureSDS
- W4502Water, Nuclease-Free Water, for Molecular BiologySDS
- A84564-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, ≥97.0% (HPLC)SDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
- R4642Ribonuclease A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)SDS
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Ox. Liq. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
Órganos de actuación
Central nervous system
Código de clase de almacenamiento
5.1A - Strongly oxidizing hazardous materials
Punto de inflamabilidad (°F)
100.4 °F
Punto de inflamabilidad (°C)
38 °C
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Genetic Framework for GATA Factor Function in Vascular Biology
Linnemann A.K., et al.
Proceedings of the National Academy of Sciences of the USA, 1, 13641-13646 (2011)
Yoon-A Kang et al.
Molecular and cellular biology, 32(1), 226-239 (2011-10-26)
Developmental and homeostatic remodeling of cellular organelles is mediated by a complex process termed autophagy. The cohort of proteins that constitute the autophagy machinery functions in a multistep biochemical pathway. Though components of the autophagy machinery are broadly expressed, autophagy
Henriette O'Geen et al.
BioTechniques, 41(5), 577-580 (2006-12-05)
A single chromatin immunoprecipitation (ChIP) sample does not provide enough DNA for hybridization to a genomic tiling array. A commonly used technique for amplifying the DNA obtained from ChIP assays is ligation-mediated PCR (LM-PCR). However; using this amplification method, we
M H Kuo et al.
Methods (San Diego, Calif.), 19(3), 425-433 (1999-12-02)
Chromatin structure plays important roles in regulating many DNA-templated processes, such as transcription, replication, and recombination. Considerable progress has recently been made in the identification of large, multisubunit complexes dedicated to these nuclear processes, all of which occur on nucleosomal
Protocolos
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
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