LB83

98062328, human blood, Lymphoblastoid

• Código UNSPSC: 41106514

Propiedades

• product name: LB83, 98062328
• origen biológico: human blood
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblastoid
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human lymphocytes, Xeroderma pigmentosum, EBV-transformed

Descripción línea celular

Established by EBV transformation of mononuclear cells from the peripheral blood of a 15 year old female. The patient has been diagnosed with Xeroderma pigmentosum (XP) complementation group G, showed no skin cancer or neurologic abnormalities and reduced NK activity. XP is a relatively rare recessively inherited disease with DNA repair deficiency. Fibroblasts from the same individual have been designated XP125LO.

Aplicación

DNA repair defect studies, hprt mutation analysis

Medio de cultivo

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Rutina de subcultivo

Maintain cultures between 3-9 x 100,000 cells/ml, dilute every 1-2 days to 3 x 100,000 cells/ml 5%

Otras notas

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