JVM-2

96090515, human unknown/unspecified, Lymphoblastoid

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: JVM-2, 96090515
• origen biológico: human unknown/unspecified
• modo de crecimiento: Suspension
• cariotipo: 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32)
• morfología: Lymphoblastoid
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Caucasian B-prolymphocytic leukaemia, EBV-transformed

Descripción línea celular

JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC.

Aplicación

Ig expression of neoplastic cells

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,13
D16S539: 12,13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
TPOX: 8,11
vWA: 17

Medio de cultivo

RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures between 3-9 x 100,000/ml; 5% CO₂; 37°C.

Otras notas

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