SSN-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96082808, fish unknown/unspecified, Fibroblast

• UNSPSC Code: 41106514
• Biological source: fish unknown/unspecified
• Growth mode: Adherent
• Karyotype: Aneuploid
• Morphology: Fibroblast
• Manufacturer/tradename: ECACC

Propiedades

• biological source: fish unknown/unspecified
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 96082808-DNA-5UG, pkg of vial of cells 96082808-1VL
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Descripción

Application

Isolation of fish virus pathogens

Biochem/physiol Actions

Fish striped snakehead fry, spontaneous production of c-type retrovirus

The fish cell line SSN-1 was initiated from whole fry tissue, Channa (Ophicephalus) striatus, commonly named ′striped snakehead′. SSN-1 spontaneously produce and release endogenous snakehead fish Mn²+- dependent C-type retrovirus. The cell line is suitable for fish virus isolation and is susceptible to epizootic ulcerative syndrome rhabdoviruses and piscine nodaviruses. SSN-1 was previously infected with mycoplasma but has been eradicated and tested negative after a minimum of 10 passages.

Preparation Note

L15 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA. Use trypsin very cautiously; 25-30°C, can be light sensitive therefore avoid prolonged exposure to bright light. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

When resuscitating a frozen ampoule of cells we recommend the cells are seeded at the higher seeding level i.e. 4 x10, 000/cm². Cells may take up to 10 days to reach 70% following resuscitation and subculture. Therefore media change flasks after 4-5 days to encourage growth.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.