P3X63Ag8U.1

94120802, mouse blood, Lymphoblast

• Código UNSPSC: 41106514

Propiedades

• product name: P3X63Ag8U.1, 94120802
• origen biológico: mouse blood
• modo de crecimiento: Suspension
• cariotipo: Not specified
• morfología: Lymphoblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse Myeloma

Descripción línea celular

P3x63Ag8U.1 is a derivative of the P3x63Ag8 cell line (Sigma Catalogue number. 85011401) from the mouse myeloma MOPC21, which was derived from a female BALB/c mouse. This variant no longer synthesises gamma-1 chains but still produces kappa light chains which are not secreted.

Aplicación

This cell line is useful for fusion to spleen cells to produce hybridomas or for hybridisation studies with other myelomas or lymphomas. Can also be used for immunoglobulin production.

Medio de cultivo

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Maintain cultures at 5-9x100,000 cells/ml; 5% CO₂; 37°C.

Otras notas

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