FGC4

94101904, rat skin, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: FGC4, 94101904
• origen biológico: rat skin
• modo de crecimiento: Adherent
• cariotipo: Modal no. 52
• morfología: Epithelial
• productos: Albumin and ß-fibrogen
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Rat hepatoma [alb-gpt plasmid]

Descripción línea celular

A subclone derived from Fao which is deficient in HGPRT (resistant to 5μg/ml 6-thioguanine and also resistant to 3mM ouabain). FGC4 contains the alb-gpt plasmid which is the bacterial gpt gene under control of the rat albumin promoter (400 bp). Produces albumin and ß-fibrinogen.GMO1

Aplicación

Gene expression studies.

Medio de cultivo

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split semi-confluent cultures (70%) 1:100 i.e. seeding at 2x1000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change three times a week.

Otras notas

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