ATHOS

93122325, Porcine thyroid, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: ATHOS, 93122325
• origen biológico: Porcine thyroid
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Epithelial
• productos: Thyroglobulin, plasminogen activator (u-PA)
• receptores: Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Porcine thyroid epithelial

Descripción línea celular

The cell line was spontaneously derived from the porcine thyroid cell line PORTHOS (Sigma Catalogue number. 93122323) after withdrawal of thyroid stimulating hormone (TSH). The cells form follicle-like structures, have a high growth rate, a high glucose consumption and are TSH independent for growth. The expression of plasminogen activators has been reported and found to be higher in ATHOS cells compared to PORTHOS cells.

Aplicación

Mechanism of thyroid follicle reconstruction

Medio de cultivo

Click:RPMI (1:1) + 10μg/ml Insulin + 10nM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 5x100,000 cells/cm² using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO₂; 37°C.

Otras notas

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