ND-C

92090913, mouse nerve, Neuronal

• Código UNSPSC: 41106514

Propiedades

• product name: ND-C, 92090913
• origen biológico: mouse nerve
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Neuronal
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse neuroblastoma x Rat neurone hybrid

Descripción línea celular

ND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.

Aplicación

Study of neuronal differentiation, cell adhesion and expression of neuronal proteins.

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm²; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO₂; 37°C.

Otras notas

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