RO82-W-1

92030502, human thyroid, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: RO82-W-1, 92030502
• origen biológico: human thyroid
• modo de crecimiento: Adherent
• cariotipo: 2n = 60-82, 82 predominant
• morfología: Epithelial
• productos: Thyroglobulin
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: metastasis
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human follicular thryoid carcinoma

Descripción línea celular

Established from the metastases of a follicular carcinoma in a female patient. Tumorigenic in nude mice. The basal release of immuno-detectable thyroglobulin increased in response to thyroid-stimulating hormone (TSH). The cell line grows as a monolayer without the formation of follicles. Electronmicroscopy studies showed the presence of microvilli with interdigitations between neighbouring cells.

Aplicación

A valuable model for the study of human follicular carcinoma.

Medio de cultivo

DMEM:Ham′s F12:MCDB 105 (2:1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Otras notas

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