NCTC 2071

90050803, mouse connective tissue, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: NCTC 2071, 90050803
• origen biológico: mouse connective tissue
• modo de crecimiento: Adherent
• cariotipo: Modal no. 63
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse C3H/An connective tissue

Descripción línea celular

Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.

Aplicación

Nutritional, immunological and toxicological studies

Medio de cultivo

NCTC 135 100% + 2mM Glutamine.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm² by scraping off the cells and resuspending into new flasks; 5% CO₂; 37°C. Freeze in 9% glycerol/91% FBS.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.