CMT 93

89111413, mouse colon, Epithelial

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: CMT 93, 89111413
• origen biológico: mouse colon
• modo de crecimiento: Adherent
• cariotipo: Hyperdiploid,modal no. 50
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse rectum carcinoma

Descripción línea celular

From a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.

Aplicación

Tumourigenicity studies

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin; 5% CO₂; 37°C. Cells grow in clumps.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.