Clone C6/36

89051705, insect larvae, Not specified

• Código UNSPSC: 41106514

Propiedades

• product name: Clone C6/36, 89051705
• origen biológico: insect larvae
• modo de crecimiento: Adherent
• cariotipo: Diploid
• morfología: Not specified
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mosquito larvae, Aedes albopictus

Descripción línea celular

Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.

Aplicación

Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.

Medio de cultivo

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO₃) level of 1.1g/l is recommended).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm² . Cells are dislodged by mechanical agitation; 5% CO₂; 28°C. Freeze in 10% glycerol.

Otras notas

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