OX-41

88051304, Reactivity: Anti-Macrophage subset, rat

• Código UNSPSC: 41106514

Propiedades

• origen biológico: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Mieloma: NS0/U
• Reactividad: Anti-Macrophage subset, rat
• técnicas: cell culture | mammalian: suitable
• isotipo: IgG2a
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse NS0/U x Mouse BALB/c spleen

Descripción línea celular

The antibody recognises a rat macrophage cell surface antigen and labels all peritoneal and 90% of alveolar macrophages. Splenic red pulp macrophages are antigen +ve, marginal zone macrophages antigen -ve. The antibody also reacts with the 120kDa antigen on granulocytes and dendritic cells and recognises rat SIRP (personal communication).

Inmunógeno

Resident peritoneal cells from (PVG-RT1c x PVG RT1u)F1 and (PVG-RT1c x DA-RT1a)F1 rats

Medio de cultivo

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

Otras notas

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Cláusula de descargo de responsabilidad

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.