This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.
Maintain cultures between 1-6x100,000 cells/ml; 5% CO2; 37°C.
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Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.
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