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Merck

C6295

Sigma-Aldrich

Cell Freezing Medium-DMSO Serum free 1x

sterile-filtered, suitable for cell culture

Sinónimos:

cryopreservation medium

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50 ML
218,00 €

218,00 €


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50 ML
218,00 €

About This Item

Código UNSPSC:
12352207
NACRES:
NA.75

218,00 €


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esterilidad

sterile-filtered

Nivel de calidad

Formulario

liquid

técnicas

cell culture | mammalian: suitable
cryopreservation: suitable

impurezas

endotoxin, tested

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

Cell freezing medium is a combination of cryoprotectant DMSO with fetal bovine serum. However serum free medium replaces serum with methylcellulose and poloxamer 188 and other additives like ascorbic acid, α-tocopherol and proline.[1]

Aplicación

Cell Freezing Medium-DMSO Serum free 1x has been used in the freezing of human neural progenitor cells (hNPCs),[2] olfactory ensheathing cells (OECs)[3] and retinal pigment epithelial (RPE) cells.[4]

Otras notas

Complete ready-to-use medium containing Minimum Essential Medium, dimethyl sulfoxide (8.7%) and methyl cellulose. Prepared according to the formulation of Waymouth[5].

Producto relacionado

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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A novel serum-free method for culturing human prenatal retinal pigment epithelial cells
Gamm DM, et al.
Investigative Ophthalmology & Visual Science, 49(2), 788-799 (2008)
Acute and delayed transplantation of olfactory ensheathing cells promote partial recovery after complete transection of the spinal cord
Lopez-Vales R, et al.
Neurobiology of Disease, 21(1), 57-68 (2006)
Chen-Yang Meng et al.
Oncology reports, 43(4), 1169-1186 (2020-04-24)
Osteosarcoma (OS) is the most common primary malignant tumor of the bone affecting children and adolescents. Chemotherapy is now considered as a standard component of OS treatment, not only for children, but also for adults. However, chemoresistance continues to pose
J Yang et al.
Oncogene, 36(34), 4828-4842 (2017-04-11)
PIM1 is a proto-oncogene, encoding a serine/threonine protein kinase that regulates cell proliferation, survival, differentiation and apoptosis. Previous reports suggest that overexpression of PIM1 can induce cellular senescence. However, the molecular mechanism underlying this process is not fully understood. Here
Xiangwen Zhan et al.
Biochemical and biophysical research communications, 501(3), 779-785 (2018-05-16)
The proto-oncogene PIM1 encodes Ser/Thr kinase and regulates cell growth, differentiation and apoptosis. However, more and more studies including ours have found that PIM1 can induce senescence in normal human diploid fibroblasts and behave as a tumor suppressor. But the

Artículos

Overview of good cell banking practices for cell line cryopreservation purposes.

Preguntas

  1. Is it possible to thaw this product, prepare aliquots and store them at -20 degrees for further use? Thank you.

    1 respuesta
    1. The stability of this material following multiple freeze/thaw cycles indicates that a fine precipitate may be evident. However, the performance of the material was is effected. While it is recommended to use this product within 5 days of the initial thaw, it would be up to the end user to continue use following refreezing. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/241/461/c6295pis.pdf

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